| AIDS is a disease with high mortality caused by HIV retrovirus.Ribonuclease H?RNase H?plays a key role in the reverse transcription of retrovirus,but cancer is another type of disease with a high mortality rate that is different from the pathogenesis of AIDS.Therefore,this paper intends to construct a biosensor for RNase H activity detection and a nano-platform for cancer-targeted photodynamic therapy,which is of great significance for improving the survival rate of patients with diseases.The main research contents are as follows:?1?Based on catalytic hairpin assembly?CHA?,a sensor was constructed for RNase H activity detection.The results showed that the response range of the sensor was from 0.001 to 50.000 U/mL,and within0.001 to 5.000 U/mL,the fluorescence intensity and concentration logarithm showed a linear correlation,and the regression equation was F=580*lg(CRH)+1958,and the lowest detection limit is estimated to be0.001 U/mL by the 3?rule.Moreover,it was found that the sensor can stably perform enzyme activity detection in complex systems and also plays an important role in inhibitor screening.?2?Based on hybridization chain reaction?HCR?and fluorescence quenching property of graphene oxide?GO?,a novel biosensor for detecting RNase H activity was constructed,which had a dynamic response range from 0.001 to 50.000 U/mL,within 0.001 to 50.000 U/mL,the fluorescence intensity and concentration logarithm showed a linear correlation,and the regression equation was F=689*lg(CRH)+2286.The lowest detection limit was estimated by the 3?rule to be 0.0007 U/mL.In addition,the sensor can be used for RNase H specific assays and had great application potential the research of anti-AIDS drugs.?3?Functionalized upconverting nanoparticles?UCNPs?for targeted photodynamic therapy?PDT?in tumor cancer cells.The results indicated that the functionalized upconverting nanoparticles have good biocompatibility and targeting,and have good diagnostic and therapeutic effects on tumor cells. |
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