Characterization And Release Behavior Of Drug Loaded Poly L-Lactic Acid Electrospun Fibers

ObjectiveA kind of surfactant with strong surface tension and low toxicity was selected to prepare biodegradable and sustained-release local drug delivery fiber.The preparation properties of the hydrophilic drugs including secnidazole and carboplatin were characterized,and the release behavior was studied through in vitro release and fitting equation,providing reference for clinical application.MethodsThe load drug in poly L-lactic acid electrospun fibrous films was prepared by electrospinning method: the drug secnidazole was homogenized by electrospinning;the drug carboplatin was treated by W/O emulsion electrospinning.Morphological structure was examined by scanning electron microscopy(SEM);X-ray diffraction(XRD)was used to observe the presence of surfactant and drug on the fiber surface;Fourier transform infrared spectroscopy(FT-IR)and differential thermal analysis(DTA)evaluated its binding state in polymer fibers.Ultraviolet spectrophotometry was established to determine the release of Tween-80 and Poloxamer188 from fibers,and the parameters such as precision,specificity and recovery rate were investigated.The HPLC method for determination of secnidazole and carboplatin content was carried out.Draw a release curve and return.ResultsWe have successfully prepared the biodegradable poly L-lactic acid electrospun fibrous films.The electrospun fibrous films obtained by using the surfactant Poloxamer188 as an additive material not only has no bead string and uniform diameter,but its percentage release rate reaches nearly 100% as the relative content increases and high output per unit time.The fiber diameter of the emulsion electrospinning is larger than that of the homogeneous solution spinning.The surface of the fiber obtained by emulsion spinning has a microporous structure.The X-ray diffraction results show that the surface of the fiber is free of carboplatin crystals,and when the content of secnidazole is 7.5% and 15%.There was no crystal precipitation on the surface when the content was 30%;The results of differential scanning calorimetry indicated that carboplatin and secnidazole were present in the fiber in an amorphous state;Fourier transform infrared spectroscopy reveals that the drug is well complexed with the fiber material.The greater the amount of surfactant complexed in the fiber,the greater the burst release rate from the fiber.The release curve conforms to the Freundlich isotherm adsorption equation,and its release behavior can be seen as a reverse adsorption process from the fiber,supporting its existence.The surface of the fiber is close to the surface of the fiber;when the amount of Poloxamer188 is compounded,the greater the amount of synenitrocarbazole released,the more the amount of secnidazole complexed in the fiber is more favorable for its release;when the compound amount of Poloxamer188 When the compounding amount of Poloxamer188 is 10%,20% and 30%,the sustained release period of carboplatin can reach 12 h,which releases the composite 0-stage kinetics and the mode of permeation of the composite core-sheath structure.ConclusionsPoloxamer188 has excellent physiological compatibility,non-toxicity and hemolytic properties and can be used for smooth renal excretion.It can be obtained by a combination of Poloxamer188 to obtain a poly-L-lactic acid electrospun fiber with a relatively uniform diameter and a bead-free structure.Different amounts of Poloxamer188 are released in different amounts,so the hydrophilic channels left on the fibers are different,so the solubilization and release behavior of other small molecules such as drugs encapsulated in the fibers are adjusted.Successfully screened the surfactant Poloxamer188 as an additive The drug-loaded poly L-lactic acid fiber membrane has a large surface area and can control release,and improves the therapeutic effect of the drug,and has a good application prospect in the local implantation of a drug delivery system in vivo.