Study On Efficiency And Mechanism Of Enhanced Hydrolysis Of Spiramycin By Synthesis Solid Acids

Antibiotic output during antibiotic production is the main source of natural antibiotic pollution,while the problem is that remental concentration in wastewater is so huge that need to be decreased.To raise hydrolysis temperature,decrease or change the solution pH,ester group,amido bond,glucosidic bond of antibiotic can be effectively hydrolyzed in certain conditions,thus effectively decrease antibiotic concentration and antibiotic potencies.Spiramycin is usually produced by fermentation process,and form preparation after separation and purifitation.This study uses three kinds of solid aicds to enhanced the hydrolysis of spiramycin production wastewater,to decrease spiramcin concentration and antibiotic potencies.we choose TiO2/SO42-super solid acids,optimize preparation conditions;the acid strenghth and totaol acidities of the solid aicds by NH3-TPD and n-butylamine titration were analyzed;effects of solid acids dosage,temperature,hydrolysis time on the kinetic of hydrolysis were studied;Staphylococcus aureus was used as test bacteria to test the antibiotic potencies of hydrolysis remnant and product The total acidities of synthetic solid acids are all above 1.10 mmol/g.After 360 min hydrolysis by Ti 15 and Ti 20,the removal rates of spiramycin were 52.7%and 61%,respectively,when the solution temperature was 35?,dosage was 1.0 g/L,spiramycin concentration was 20 mg/L,and the removal rate sof antibiotic potency were 69%and 71%,respectively,it can be seen that there is an obvious positive correlation between removal rates of sp;iramycin concentration/antibiotic potency and total acidity of solid acid.Hydrolysis efficiency can be enhanced by raise the solution temperature;mycarose which was the important pharmacodynamics of spiramycin was lost during hydrolysis,which leads to the decrease of antibiotic potency.When temperature was 35?,solid acid dosage was 20 g/L,spiramycin concentration in the 433 mg/L spiramycin production wastewater decreased 58.8%and 99%by the enhaced hydrolysis of Ti 15 and Ti 20,respectively,and antbiotic potencies decreased 56.2%and 94.8%,respectively.As for heteropoly acids,the results show that the three heteropoly acids has total acidity of 1.1-1.25 mmol/g,the removal abities of three kinds of hteropoly acids follow an order of H3[P(W3O10)4]>H4[Si(W3O10)4]>H3[P(Mo3O10)4].When solution temperature was 35? solid acid dosage was 1.0 g/L,after 360 min hydrolyzed by H4[Si(W3O10)4],the 20 mg/L spiramycin and its antibiotic potency can be totally removed.Mycarose and forosamine both get lost during hydrolysis of the three kinds of heteropoly acids.When temperature was 35? solid acid dosage was 10 g/L,spiramycin concentration in the 433 mg/L spiramycin production wastewater can be totally decreased by the 60 min-hydrolysis of H4[Si(W3O10)4].As for SiO2/SO3H solid acids,the preparation conditions were optimized,total acidity was tested,the effct of solid acid dosage,temperature and hydrolysis time on hydrolysis kinetics were studied,changes on antibiotic potencies was analyzed.The best preparation condition of 4-ISS-600 and the best hydrolysis condition of 35?,1.0 g/L were chosen.Prepared solid acids have total acidity of 1.1-1.25 mmol/g.At the best hydrolysis condition,the 20 mg/L spiramycin can be totally removed by the enhanced hydrolysis of 4-ISS-600 SiO2/SO3H solid acid,antibiotic potency can decrease 40%.When temperature was 35?,solid acid dosage was 10 g/L,60%of spiramycin concentration in the 433 mg/L spiramycin production wastewater can be totally decreased by the 180 min-hydrolysis of 4-ISS-600 Si02/S03H solid acidThe research of this paper put forward a pretreatment method of antibiotic production wastewater which can be applied in mild conditions,acid features and hydrolysis effects can be raised by optimize preparation conditions of solid acids,hydrolysis effects can be raised by optimize hydrolysis conditions,antibiotic concentration,antibiotic potency and inhibitory to methanogens can decrease effectively.This work can provide a promising physical and chemical method of antibiotic production wastewater pretreatment,effective technical support of antibiotic wastewater treatment can be provided.