PEGylated Liposomes Coated Alumina Nanoparticles As An Effective Vaccine Adjuvant-Delivery System

As is known to all,lymph node is the key point of vaccine targets,where antigen presentation and booting of T-cell-mediated immune responses occur primarily.However,the traditional vaccine formulations were absorbed easily by APCs at the injection site,where cannot induce effective immune response.We performed to the rational design of vaccine vehicles based on alumina nanoparticles as PEGylation to achieve target lymph node,where a lot of antigen presenting cells,especially dendritic cells(DCs).Objective:we aimed to investigate the immune efficacy of OVA-ANs,OVA-PLANs,OVA-PEG-PLANs after subcutaneous vaccination in mice compared to traditional aluminum adjuvants.Method:We loaded the liposome with alumina nanoparticles(ANs)by thin film hydration method to form ANs liposome(PLANs)and modified the surface of liposome with polyethylene glycol,just PEG-PLANs.All formulations(ANs,PLANs and PEG-PLANs)were uptake by APCs well and escaped from lysosomal.In the body,after mice were given subcutaneously with different vaccine prescription,PEG-PLANs were first arrived in lymph node and most abundant in lymph node.Three weeks later,the immunogenicity was assessed by ELISA.Results:FTIR and TEM proved the formation of OVA-ANs and OVA-PLANs.Different vaccine vectors are ingested in addition to AM.LSCM showed the different vaccine carriers all escaped from endo/lysosomal in addition to AM.PEG-PLANs first migrated to the lymph nodes and induced powerful immune responses.Conclusion:OVA-PEG-PLANs induced powerful immune responses in vivo,including production of antigen-specific antibodies and a significant increase in CD8~+T cells.PEG-PLANs can as an effective Vaccine Adjuvant-Delivery System(VADS).