Synthesis And Properties Of Thioacetal Fluorescent Probes Base On Phenothiazine Derivative

With the improvement of living standards,more and more attention was paied by people to health issues.Environmental pollution is becoming more and more serious,and one of the most serious problem is water pollution.Many methods have been designed to detect the pollution of heavy metal ions in water.Fluorescent probes are simple and efficient methods used to detect heavy metals.Most fluorescent probes are based on the enhancement and attenuation of fluorescence to detect metal ions.The accuracy of the detection results is easily affected by external environments.Therefore,more efficient detection methods are necessarily to be explored.In this paper,three kinds of fluorescent probes based on phenothiazine derivatives with high sensitivity and high selectivity for Hg²⁺ions detection were designed.The detection mechanism of the probes was deprotection reaction of thioaldehyde.The aldehyde group on the phenothiazine reacted with thiol to form corresponding thioaldehyde,and then deprotected with Hg²⁺in aqueous solution to form a corresponding aldehyde group.Thereby,fluorescence was enhanced to achieve the purpose of the Hg²⁺detection,which accompanied with an obvious color change from colorless to light yellow.The Hg²⁺in the aqueous solution could be detected with naked eye by color change.?1?2-?1,3 dithiolane?-10-ethylpHenothiazine was synthesized by phenothiazine and 1,3-ethanedithiol.Infrared spectroscopy,nuclear magnetic resonance spectroscopy and elemental analysis and other methods were used to characterize and analyze its structure.The ability of Hg²⁺detection was analyzed by UV-visible spectroscopy and fluorescence emission spectroscopy.The response time,pH influence,anti-interference ability and actual detection ability were analyzed and studied.?2?2-?1,3dithiane?-10-ethylpHenothiazine was synthesized by phenothiazine and1,3-propanedithiol.The structure was characterized and analyzed by various methods as that mensioned above,the ability of Hg²⁺detection was analyzed by UV-visible spectroscopy and fluorescence emission spectroscopy,and its response time,pH influence,anti-interference ability and actual detection ability were studied.?3?2-?Diethylthio?-methine-10-ethylphenothiazine was synthesized by phenothia-zine and ethanethiol.The structure was characterized and analyzed by various methods as that mensioned above.The UV-visible spectroscopy and fluorescence emission spectroscopy were measured to analyze the ability of Hg²⁺detection.The response time,pH influence,anti-interference ability,actual detection ability and color application were studied.The innovations of this paper:?1?Three thioacetal fluorescent probes based on phenothiazine derivatives were designed and synthesized.The results show that the compounds have high selectivity,high sensitivity,short response time,strong anti-interference ability.It can be used for Hg²⁺detection with wide pH range and solid colorimetric reagent.?2?Three kinds of fluorescent probes detect Hg²⁺based on deprotection reaction,we can observe the color changes with naked eye to confirm the exist of Hg²⁺.The red shift of UV absorption spectrum and fluorescence emission spectrum make the results more accurate.