Study On Techonology Of Preparing High Fructrose Syrup From Sugarcane Final Molassess

The sugarcane final molasses is a major by-product of the sugar industry and a good source of sugar.Sugar cane plants typically use molasses for fermentation to produce alcohol,which reduces the economic value and market adaptability of molasses.Therefore,it is necessary to develop a new high-value utilization method of sugar cane final molasses to improve the economic benefits of the traditional sugar industry.In this study,high fructose syrup was prepared from sugarcane final molasses by means of membrane separation,ion exchange,solid acid catalyzed hydrolysis and enzymatic catalysis,in order to provide a new reference for the high value utilization of sugarcane final molasses.The research work is as follows(1)In view of the shortcomings of the traditional sugar industry for the analysis of molasses,the Roe colorimetric method,3,5-dinitrosalicylic acid method and resorcinol method,which are more suitable for the study,were respectively used for the determination of sucrose,reducing sugar and fructose in molasses.And other physical and chemical indicators of the molasses used were also determined to provide a reference for subsequent purification experiments(2)The molasses was cleaned by membrane method,and the results showed that the turbidity removal rate of the micro-filtered molasses after 5 times dilution was the best(>99.2%).The 8 kDa ultrafiltration membrane has the best ultrafiltration effect.And the optimum transmembrane pressure and operating time were 0.16 MPa and 70 min,respectively,and the corresponding total sugar loss rate is only 3.6%.Four empirical models were used to fit the changes in membrane flux during the ultrafiltration process.The results showed that the filter cake formation model had the best fitting effect(R2=0.97926)(3)The ion exchange resin was used for further clarification.The results showed that when the volume ratio of HZ016 and D202 resin is 1:1,the feed volume is 50 mL,and the feed flow rate is 1.5 BV/h,the desalting and decolorization effect is the best.The cleaned sample showed a significant decrease in conductivity.UV-visible spectroscopy and particle size analysis indicated that the absorbance of the sample was greatly reduced,and the macromolecular impurities(100?1000 nm)have been substantially removed(4)The treated molasses is concentrated to obtain a liquid sugar.The solid syrup was hydrolyzed by strong acid resin to obtain the conversion syrup,and the reaction conditions were studied.Taking sucrose conversion rate and reducing sugar yield as indicators,and the optimum process parameters were obtained by single factor and orthogonal experiment:resin addition amount 4 mL(10 mL sugar solution),temperature 80?,reaction time 30 min.And the content of fructose and glucose in the sample after acid hydrolysis was 14.5%and 30.8%,respectively.(5)The primary high fructose syrup was prepared from the converted syrup by isomerization using glucose isomerase.Taking fructose content as an indicator,and the single factor experiment determined the range of reaction conditions and the main influencing factors,and then optimized by response surface experiments.The results showed that the optimal process parameters for enzymatic isomerization were:reaction temperature 60?,enzyme dosage 1.41%,reaction time 18.3 h.The fructose content of the reacted sample was 33.8%.(6)High fructose syrup was prepared by vacuum concentration,and the analysis results showed its physical and chemical indexes basically met the requirements of GB/T 20882-2007.Finally,taking the processing of 100 tons of sugarcane final molasses as an example,the economic benefits of the process were estimated and analyzed.The results showed that it has good economic benefits and development prospects,which can provide reference for the high value utilization of sugarcane final molasses and secondary entrepreneurship.