Effects Of Adding Different Microbial Agents On The Corn Stalks Silage Microflora And Quality

China is a big corn producer,and it produces a lot of corn stalks while producing corn.These straws have not been rationally utilized at present,resulting in waste of resources,environmental pollution and certain impact on crops.In recent years,micro-storage technology has been paid more and more attention in the utilization of straw.Microbial fermentation in micro-storage process is the key to corn stalk feed fermentation,which directly affects the quality of micro-storage.Therefore,this study used the current effective method and High-throughput sequencing Miseq platform to study the effects of three different microbial agents on the nutritional quality and microbial structure of corn straw micro-storage,and studied the nutritional quality during micro-storage.Correlation of bacterial structure.The main results and conclusions are as follows:(1)The addition of W microbial agent(Lactobacillus plantarum+Bacillus methyltrophicus+Meyerozyma)played an important role in increasing the content of reducing sugar and reducing the content of crude fiber in microstored corn straw.The content of reducing sugar was 3.5 times as much as that in the control group,and the content of crude fiber was 20.54%lower than that in the control group.The correlation analysis showed that there were 8 genera negatively correlated with crude fiber content and 3 genera positively correlated with reducing sugar content.Microbial agent W improved the abundance and diversity of microorganisms in microstored corn straw.At the phylum level,microbial agent W significantly increased the relative abundance of Firmicutes during the whole microstored process,as well as the initial relative abundance of Proteobacteria.At the family level,microbial agent W changed the initial bacterial community composition of straw and the change rule of Lactobacillus family during microstored.(2)The crude protein content of microbial straw was increased by 23.1%by adding microbial agent S(Bacillus methyltrophicus+Lactobacillus plantarum)compared with the control group.Four genera were found to be significantly and positively correlated with crude protein by correlation analysis.Four genera were found to be significantly and positively correlated with crude protein by correlation analysis.Microbial agent S improved the abundance and diversity of microorganisms in microstored corn straw.At the phylum level,the microbial agent S significantly increased the relative abundance of the whole microstored process of the Proteobacteria;At the level of family and genus,the addition of microbial agent S changed the composition of bacterial community during the whole microstored process.There was no obvious dominant bacteria in the whole process of microstored,and the flora of each family and each genus was relatively balanced.(3)The addition of microbial agent Y(Compound strains of lactic acid bacteria and cellulose-degrading bacteria)increased the total acid content in the microstored corn straw,which was increased by 14.7%compared with the control group.One genus was positively correlated with total acid content.Microbial agent Y improves the diversity of microorganisms in microstored corn straw and reduces the abundance of microorganisms.At the phylum level,microbial agent Y significantly increased the relative abundance of Proteobacteria during the whole microstored process.At the family level,the initial dominant bacteria of bacterial community were changed by adding microbial agent Y;at the genus level,Lactobacillus was dominant in the process of straw microstored with Y microbial agent,but its dominance was smaller than that of the control group.