Research On MIPs Sensor Array Design,Green Synthesis And Its Application

The chemical sensor array is sensor integrated combination with high recognition ability and high accuracy.As a new type of artificial biomimetic receptor,molecularly imprinted polymers(MIPs)are widely used in a variety of fields.The MIPs material is integrated into a sensing array that takes full advantage of the multi-selective differences in MIPs materials.In addition,the sensor array platform integrates multiple independent MIPs to overcome the limitations of high cross-reactivity and low affinity for a single MIP material.In this paper,the MIPs were quickly and low-carbon constructed by photo-activated RAFT miniemulsion polymerization at room temperature,and integrated multi-channel MIPs sensing array,the "fluorescent dye replacement strategy" was adopted,used Rhodamine B as a fluorescent probe to investigate the ability of the sensor array for the identification of template molecules and non-template molecules and the identification of 2,4-dichlorophenoxyacetic acid in actual samples.The main work of this paper is as follows:(1)Preparation and characterization of MIPs and NIPs: 2,4-D MIPs(P1),2,4-DCPA MIPs(P2),POAc MIPs(P3)and NIPs(P4)were successfully prepared by photoactivated room-temperature RAFT miniemulsion polymerization technology.The chemical composition,surface morphology,particle size,specific surface area,pore size and distribution of each polymer(P0-P3)were investigated by means of Fourier transform infrared spectroscopy,Scanning electron microscopy,Malvern laser particle size analyzer,specific surface and pore size analyzer.In addition,the response behavior of MIPs to different imprinted molecules and non-imprinted molecules at a fixed concentration was examined.(2)MIPs sensing arrays recognize template molecules and non-template molecules: Rhodamine B was used as fluorescent probe to explore the ability of MIPs fluorescence sensing arrays to distinguish and identify imprinted(2,4-D,2,4-DCPA,POAc)and non-imprinted(benzoic acid)molecules in acetonitrile and pure water.The obtained data was analyzed in depth by principal component analysis(PCA)in the pattern recognition method.In the acetonitrile solution,the PCA plot showed that the cumulative scores of the first two principal components reached 98.12%;in the aqueous solution,the PCA plot showed that the cumulative scores of the first two principal components reached 99.59%.All of the above indicate that the sensing array can successfully capture template and non-template molecular features in acetonitrile and water.Further,the cluster analysis method was used to distinguish the template molecules from the non-template molecules in the two systems of acetonitrile and water.The results were automatically aggregated into 4 classes with an accuracy of 100%.Studies have shown that the MIPs sensing array can accurately identify template molecules and non-template molecules.(3)MIPs sensing arrays identify 2,4-D in complex samples: Rhodamine B was used as a fluorescent probe to explore the ability of MIPs fluorescence sensing arrays to identify 2,4-D in natural waters and mixed juices.The PCA chart shows that the first two principal components in natural water are 98.51% cumulatively;the first two principal components in the mixed juice are 96.44%.This indicates that the MIPs fluorescence sensing array can capture the digital features of 2,4-D in natural water and mixed juice.In summary,this paper establishes a method for visible light activated RAFT miniemulsion polymerization of green low carbon and rapid construction of imprinted polymer.This not only expands the application of visible light activated room temperature RAFT polymerization in the field of sensing arrays,but also provides new methods and new technologies for rapid detection of organic pesticides.