The Preventative Effect Of Lactobacillus Plantarum Microcapsule And Its Wall Material On Diet Induced Obesity In Mice

Nowadays,owing to people's unhealthy dietary habits and irregular lifestyle,there are a series of metabolic diseases related to obesity such as diabetes,hypertension,and atherosclerosis which are serious public health issues affecting human physical conditions.Therefore,it is of great significance to search and develop one kind of functional foods with weight-loss and lipid-lowering properties.Dietary fiber played an important role in maintaining people's health condition,and the intestinal flora fermented the fiber to produce functional substances which were the basis for regulating their metabolic balance.Therefore,functional foods with dietary fiber?especially prebiotics?and probiotics can be effective to improve people's health conditions.However,Lactobacillus plantarum is vulnerable to the acidic environment of the digestive tract,which to a large extent limits its beneficial effects on the host.Microencapsulation of probiotics is currently a viable and effective method to protect their activity.Lactobacillus plantarum as a probiotic was encapsulated by using arabinoxylan?prebiotics?as the wall material in this subject to explore and analyze the preventative effect of probiotics and prebiotics on obesity in mice collaboratively.The microcapsules of Lactobacillus plantarum were prepared by using alginate and arabinoxylan as wall materials.Based on this,the animal model of mice was employed to explore and elaborate the synergistic effect of probiotics and wall material?arabinoxylan?on preventing obesity in mice and the possible effect of intestinal flora on it.The main research contents and results are as follows:1.Extraction and preparation of arabinoxylan from cereal bran.Arabinoxylan was extracted from wheat bran by the method of alkaline-hydrogen peroxide.The content of ferulic acid in arabinoxylan is 9.15?g/mg,and the monosaccharide composition is as follows:the contents of xylose,arabinoxylan,glucose and galactose are 53.85%,35.30%,3.65%,and 1.90%,respectively.The ratio of A/X is 0.65.2.The preparation and characterization of Lactobacillus plantarum microcapsules.Lactobacillus plantarum microcapsules were prepared via the sharp-hole coagulation technique using arabinoxylan?including arabinoxylooligosaccharides?and sodium alginate as wall materials and Lactobacillus plantarum as core material.The experimental results showed that the encapsulation yield is 73.60%±0.01%,the embedding efficiency is 81.27%±0.17%,and the effective load is?3.68±0.4?×10¹¹ cfu/g,respectively.Moreover,the results from the acid resistance test showed that the number of bacteria loaded in microcapsules decreased by only1.2 logarithms,whereas the bacterial suspension decreased by 6.4 logarithms.It also demonstrated that microencapsulated Lactobacillus plantarum were released completely with1 h in the intestinal release test.And the number of bacteria loaded in microcapsules decreased by only 0.55 logarithm whereas the bacterial suspension decreased by 5.6 logarithms in the gastrointestinal tests.Additionally,after the microcapsules were stored at-20°C,4°C and 28°C for 7 weeks,the number of bacteria decreased by 1.2,1.7 and 5.2 logarithms,respectively.In summary,the prepared microcapsules could protect the Lactobacillus plantarum and could be used as a vehicle to deliver the probiotics to the target location in the gastrointestinal tract.3.The preventative effect of Lactobacillus plantarum microcapsules on the mice obesity induced by high fat diet.The results from Lactobacillus plantarum microcapsule and microcapsule group showed that they were significantly lower in terms of the weight gain,body fat ratio of the mice in comparison with high-fat group.Moreover,they could also effectively reduce the content of fat in liver and inhibit the bulk increase of white fat cells.And it was observed that compared to high-fat group,antioxidant capacity of liver tissue was improved and the content of lipid peroxidation products was reduced in Lactobacillus plantarum microcapsule and microcapsule group.Additionally,the results from the lipid metabolic experiments indicated that there are some increase on the activity of lipoprotein lipase,hepatic lipase and total lipase in liver,which to a large extent accelerated the catabolism of adipose tissue in Lactobacillus plantarum microcapsule group.In addition,in the reverse transcription-polymerase chain reaction experiments,it is confirmed that the gene expression of fatty acid synthase?FAS?is down whereas the transcription factor peroxisome proliferator-activated receptor?PPAR-??was up which made the equilibrium between the synthesis and oxidation of fat partial to oxidative pathway,indicating that they had the anti-obesity effect.Based on the high-throughput sequencing analysis of mice intestinal flora,it was found that the abundance of rumen,streptococcus and digestive streptococci at the family level was reduced in Lactobacillus plantarum microcapsule group,the rikenellaceae and enterococcaceae were improved which manifested that dietary intervention could improve intestinal flora disorder caused by high-fat diet.To summarize,composite encapsulation technique was employed to prepare Lactobacillus plantarum microcapsule,which possessed good encapsulation effects,gastrointestinal tolerance and storage stability properties.Meanwhile,it was capable of promoting weight-loss and preventing the development of obesity of mice,and had to some extent regulatory effect on intestinal flora disorder caused by high-fat diet to enhance host's healthy conditions finally.Therefore,this subject provided a theoretical basis for how to improve people's health conditions by utilizing the dietary fiber and probiotics collaboratively...