Research Of Logic System Based On Nanoparticles And Enzyme

Along with the rapid development of computer science and technology,computers are increasingly used to calculate mathematical problems in various fields of science and technology,such as mechanics,physics,chemistry,biology,economics,etc.However,the scientific problems are going to more complicated,and the performance of traditional electronic computers is gradually unable to meet the demand.The field of emerging computing such as quantum computing,photon computing,and Nano-computing is flourishing,the DNA Nano-computing is a promising field.DNA are widely used in Nano-computing,owing to their natural micro-molecular-specific effects,is play a pivotal role in the development of new high-performance computing.The molecular calculation combined with inorganic nanomaterials and biomolecules has made breakthroughs in complex computing,information processing,nanomachines,drug delivery,and molecular detection.In this paper,DNA molecular logic gates and DNA nanomachines were designed.NUPACK and other softwares are used to design the DNA coding of the computational model,combined with enzyme(endonuclease),nanoparticles,DNA self-assembly technology and strand replacement technology,the multi-level study on the DNA calculation and logic system was carried out.Firstly,molecular calculation logic gate based on the self-assembly of AuNPs was realized.In this molecular computing model,we have successfully designed and implemented a programmable strategy that can control the assembly of AuNPs through mono-enzyme or multi-enzyme as input signal.The AuNPs disassociation as output signal confirmed by agarose gel electrophoresis.A set of DNA logic gates(YES,AND,OR)were established based on this stratedy.Secondly,we have researched the DNA Nano machines powered by enzyme.DNA walker strand and DNA path strand are modified on the same AuNP.The DNA walker is designed to have a nicking recognition sequence,and the DNA path strand is designed to have a complementary nicking cleavage sequence.In the presence of the nicking enzyme,the DNA walker strand cleaving the DNA path strand along the AuNP surface,then we have build a DNA Nano machines.Through the work of this study,a logic gate("YES")was build,enzyme as the input signal differen number of bases as influences factor,By hybridizing with small-sized gold nanoparticles and by means of multiple detection methods such as agarose gel electrophoresis and electron microscopy,the final structure was observed and the calculation results were analyzed.Finally,we propsed a light controlled information encryption system model.This system model uses a two-dimensional DNA origami structure as information coding platform,and is connected with coding site by functional AuNPs to realize the coverage encrytion of the plaintext encrypted information.The decryption of the information is done by DNA key and UV light.Information extraction and recognition can be achieved by means of transmission electron microscopy and atomic force microscopy.