H₂S Fluorescent Probe Based On Naphthalimide Derivative

Hydrogen sulfide?H₂S?is a colorless,flammable gas inorganic compound under normal conditions.When its concentration is low,it will have a odor similar to that of rotten eggs.However,when the concentration is high,the odor will disappear.Although the past understanding of it is only toxic gas,but after research found that its role can not be underestimated.For example,industrial production of sulphur,identification of some metal ions by reaction,medical treatment of some diseases and regulation of the nervous system,etc.Therefore,fluorescent probe technology has been widely used in the detection of pharmacodynamics,biological detection,targeted drug delivery of some cells and so on.Fluorescent probes have the advantages of large conjugated pi bond structure and rigid planar structure,which make them have high fluorescence quantum yield,good selectivity,high sensitivity and so on.Fluorescent probes are not affected by external electromagnetic fields when used to detect the substances to be measured.Fluorescence imaging technology has the advantages of real-time and non-invasive,so it is very useful to detect H₂S in living cells.In the third chapter,we use 4-bromo-1,8-naphthalene divalent anhydride as fluorescent group and 4-naphthalene ring as modified linked azide group as recognition group for H₂S.The synthesized probe solution was dripped into DSMO-PBS?10 m M,p H 7.4,8:2,v/v?system and showed weak fluorescence at 542 nm.The fluorescence of the system increased rapidly after adding H₂S.The probe has good specific recognition function,high sensitivity and low detection limit.It can be used to detect H₂S in living cells.In the third chapter,we designed and synthesized a fluorescent probe 3-1 based on naphthalimide derivatives,which has symmetrical structure and double recognition sites.With the increase of H₂S concentration,the fluorescence intensity of 3-1 at 532 nm increased significantly.In addition,the probe exhibits excellent selectivity and fast response time.More importantly,3-1 can be used to detect endogenous and exogenous H₂S in living cells.