Development And Research Of Health Food Dendrobium Nobile Geqi Pills

The rich genuine traditional Chinese medicine Chizhou Dendrobium nobile has been bred in the superior nature environment of the Guizhou,which has become the main economic crop in the region.But it is mainly sold in the primary processing of low added value.This subject mainly focuses on health food with Dendrobium nobile as the main raw material,to increase additional value of Guizhou Chishui Dendrobium nobile and to boost local economic development.On the basis of the theory of traditional Chinese medicine,according to the prescription of load and drawing lessons from the modern food science and the evaluation methods of toxicology test,the Dendrobium nobile Geqi Pill was prepared by four chinese herbals,such as Dendrobium nobile,medlar,radix puerariae and schisandra chinensis.The product can enhance immunity and has auxiliary protective effect on chemical liver injury.And it is mainly suitable for the crowd with low immunity and chemical liver injury risk.This paper detailedly expounded the prescription basis of Dendrobium nobile Geqi pill and roundly researched the preparation process,quality standard,stability and safety.The prescription of Dendrobium nobile Geqi pill are Dendrobium nobile2.4g,medlar3.6g,radix puerariae3.6g and schisandra chinensis1.2g,which is determined according to the basis of classical prescriptions of TCM and the existing research results.In the experiment,on the basis of the technical characteristics,the properties of the materials as well as the function and treatment of the preparation,taking with the dendrobine,crude polysacchride content and the yield of dry extract as indexes,using the orthogonal experiment design and the method comprehensive score,the extracting process of the Dendrobium nobile,medlar,schisandra chinensis,which are mixed,could be divided into alcohol extraction and water extraction.The preparation conditions can be optimized to be that using 95% ethanol thoroughly soaking to the heart,Adding 10 times of 95% ethanol and refluxing for 2 hours,and filtering and collecting the filtrate,the extraction repeating 2 times.And than combined the filtrate,concentrated to a relative density of 1.15(60?,-0.07 MPa ~-0.08MPa).The residue was decoctting 2 times with 10 times of water,each time 1 hours,concentrated to a relative density of 1.15(60?,-0.07 MPa ~-0.08MPa).Then the ethanol extract and water extract were combined,depression drying,crushing into 120 mesh screen.Adding the radix puerariae of 120 mesh and then combining fully,with 70% ethanol as wetting agent,made the soft material and plodding,cutting and rubbing pill,finally drying(55?,-0.08 Mpa,12hours).In the trial production of three batches of samples,the average yield of the three batches of samples reached 96.4%.Through detecting the physical and chemical indicators,microbial indicators and signs of components of Dendrobium nobile Geqi pill,and referring to GB 16740,GB 2763,GB 29921 and the the people's Republic of China Pharmacopoeia,the quality standard of Dendrobium nobile Ge qi pill was initially established.Then aiming at detection methods of Dendrobium nobile pill crude polysaccharide,there was a methodological study.According to the technical requirements,the draft of Dendrobium nobile Geqi pill quality standards text was made.In quality standards,sign components of Dendrobium nobile Geqi pill were provided as follows: crude polysaccharide(glucose)is more than or equal to 2.0(g / 100g),puerarin is more than or equal to 1.5(g / 100g).Dendrobium nobile Geqi pill has a stable quality.Under temperature of 37±2? and relative humidity of 75 ± 5%,it can be stored for three months.The efficacy ingredients that are crude polysaccharide and puerarin maintained stability.The health indicators remained unchangeably.the indicators still comply with the provisions,therefore the provisional product shelf-life is 24 months.It was found that the rat and mouse acute oral toxicity test result MTD of Dendrobium nobile Geqi pill was greater than 15000mg/kg.BW.According to the classification of acute toxicity,it belonged to nontoxic.It Was shown that Dendrobium nobile Geqi pill has no mutagenic effect on human by Ames experiment,bone marrow cell microkemel experiment and mouse sperm malformation experiment.It was also found that rats body weight,feeding amount and weekly food utilization rate,the ratio of internal organs heavy,blood biochemical criterion and the end of the blood biochemical index test results as well as histopathological examination after 30 days feeding experiment,compared with the control group,which were in the normal range.The safety test showed that Dendrobium nobile Geqi pills can be eaten by people,which are all safe...