Construction And Application Of Chemiluminescence Resonance Energy Transfer Double Receptors System

Chemiluminescence resonance energy transfer?CRET?,unlike fluorescence resonance energy transfer,does not require external excitation sources.Essentially,it is a non-radiative energy transfer process that transfers the energy generated by chemiluminescence reaction from energy donor to energy acceptor.Moreover,chemiluminescence has the characteristics of high efficiency,high sensitivity and no background light interference.It is usually used to detect trace substances.In recent years,graphene nanomaterials have attracted the attention of researchers from all walks of life.Graphene oxide,which comes from chemically modified graphene,has a large number of oxygen-containing groups on its surface,and has the advantages of good dispersion in water and large specific surface area.It is commonly used in sensor and drug delivery.In chemiluminescence system,it is often used as an energy acceptor,which can lead to fluorescence quenching.In addition,nitrogen-doped graphene oxide has better optical properties and stability than graphene oxide because it contains amide,carboxyl and hydroxyl groups on its surface.At the same time,nitrogen-doped graphene nanosheets can be used as energy acceptors in chemiluminescence system because of their excellent fluorescence properties and easy surface modification.Furthermore,it was found that nitrogen-doped graphene nanosheets could enhance the sensitivity of TCPO-H₂O₂ chemiluminescence system.Luminescent hydrogels,which emit under ultraviolet or visible light and can continue to emit after stopping irradiation,have attracted wide attention for their potential applications in sensing imaging,fluorescent probes,optical switches and other fields.At present,most chemiluminescence studies focus on flash luminescence,while hydrogels containing long afterglow materials can carry out long-term luminescence,improve the flash luminescence time is short,and expand the application scope of chemiluminescence.Different long afterglow materials have different afterglow properties.Some long afterglow materials can last up to 2000 minutes or even longer.Here,this paper mainly studies the following aspects:?1?Fluorescein nanoparticles were prepared by surfactant-assisted-reprecipitation method.Nitrogen-doped graphene oxide nanosheets were prepared by hydrothermal method using citric acid and ammonia as raw materials.The fluorescein nanoparticles were characterized by scanning electron microscopy,ultraviolet spectrophotometer,phosphorescence/fluorescence spectrophotometer,Fourier transform infrared spectroscopy,transmission electron microscopy and XPS photoelectron spectrometer.Characterization and optical properties were studied.Then,based on TCPO-H₂O₂ as energy donor,fluorescein nanoparticles and N-GONs as energy acceptor,a chemiluminescence resonance energy transfer double acceptor system was constructed,and a new detection method for hydroquinone was established.The detection limit of this method was 0.03 nmol L^-1?3??,and the linear range was 0.1-80 nmol L^-1.?2?Using TCPO-H₂O₂ as energy donor,graphene oxide nanosheets and FAM-ssDNA as energy acceptor,a chemiluminescence resonance energy double acceptor system was constructed,and microRNA-21 was detected with high sensitivity and selectivity by amplifying the signal by DSN enzyme cycle.The experimental results show that the detection limit of this method is 1.1 x 10^-1414 mol L^-11 and the relative standard deviation?RSD?is 4.0%(the concentration of microRNA21 is 1.0 pmol L^-1).?3?A chemiluminescence detection and analysis platform was constructed by combining long afterglow luminescent material(SrAl₂O₄:Eu²⁺,Dy³⁺)with chitosan hydrogel and adding copper ions to the system.A new method for detecting glutathione was established.The linear range was 0.01-8?mol L^-1,and the detection limit was 3.3 nmol L^-1?3??.