Luminescent Nanomaterials Developed Sensing And Detection Of Tumor Markers

Malignant tumor is one of the main threats to human lives and health.It is of great significance to accurately detect the concentration of tumor markers for the prevention of malignant tumor,early screening and postoperative recurrence.In recent years,the construction of sensors based on luminescent nanomaterials for detection of the concentration of tumor markers has attracted more and more attentions.In this paper,cadmium telluride quantum dots?CdTe QDs?and persistence luminescent nanomaterials are selected as luminescent materials to design a nanosensor that can be used for single/double detection of tumor markers.This sensor can not only be used for the detection of serum and other practical samples,but also has a bright application prospect in bioimaging.The main research results of this paper are listed as follows:1.The emission wavelength of CdTe QDs can be controlled by adjusting the time of heating reaction.CdTe QDs,emitted at 590 nm and 731 nm,were respectively loaded on the surface of mesoporous silica nanoparticles to amplify the fluorescence signals.They were modified with aptamers to prepared fluorescent probes that can specifically recognize carcino-embryonic antigen?CEA?and prostate specific antigen?PSA?.Molybdenum disulfide nanosheets?MoS₂ NSs?is used as the energy acceptor in the sensing system because of its strong quenching ability and adsorption of single-strand DNA?ssDNA?.Based on the above,a fluorescence aptasensor based on the synergistic effect of fluorescence resonance energy transfer?FRET?and inner filter effect?IFE?has been designed for dual detection of CEA and PSA.2.To improve biocompatibility,blue emitting ZGO:Mo NRs with low toxicity was employed to construct the luminescent probe,and the Au@Ag@SiO₂ NPs was used as the quenching agent because their absorption spectrum occurs at the range of blue emitting.in virtue of DNA complementary hybridization technology,a highly sensitive and specific aptasensor was developed for PSA detection.The linear relationship of this method ranged 10pg ml^-11 from 10 ng ml^-1,and the limit of detection?LOD?of PSA is 9.2 pg ml^-1.3.One dimensional persistence luminescent nanorods?PLNRs?were synthesized by doping Mn,Mo,Cr and Sr into zinc germaniate?Zn₂GeO₄?matrix materials,respectively,which were denoted as ZGO:Mn NRs,ZGO:Mo NRs,ZGO:Cr NRs and ZGO:Sr NRs.The green emitting of ZGO:Mn NRs and the near infrared?NIR?emitting of persistence luminescent nanoparticles ZGC NPs were used to construct luminescent probes.Polydopamine nanoparticles?PDANSs?were selected as quenching agents.A method based on FRET mechanism was designed for dual detection of PSA and CEA.Taking detection of CEA as an example,this method can be applied in bioimaging to achieve in situ monitoring of CEA in HeLa cells.These indicates that the analysis method based on persistence luminescent nanomaterials with different morphologies has a bright application prospect in screening and treatment of tumor diseases.4.The blue emitting ZGO:Sr NRs were used as the detection signal.ZGC@PDA NPs was prepared by coating polydopamine?PDA?on the surface of ZGC NPs.NIR emitting ZGC@PDA can not only quench luminescence signal but adsorb ssDNA,which can be used as a reference signal and quenching agent in detection system.A ratiometric luminescent aptasensor was established to monitor the level of CEA with the LOD of 0.36 pg ml^-1.This method shows good luminescence stability and excellent selectivity,which is suitable for the accurate detection of tumor markers in actual samples.