Paper-based Artificial Antibody Electrochemical Biosensor Based On Metal Oxide Material

In recent years,electrochemical analysis has attracted widespread attention and application due to its high sensitivity,low signal-to-noise ratio,and simple operation.In this thesis,electrochemical analytical method are combined with biosensors to construct electrochemical biosensors for achieving simple,rapid and sensitive detection of biomolecules.However,since the lower concentration of the target and more interfering substance in the complex sample,the detection effect has been affected to some extent.It is necessary to develop a sensor with high sensitivity and selectivity.Therefore,a molecular imprinting as an artificial antibody is introduce into the sensor in order to achieve specific recognition toward the target molecule.In this paper,combined with the microfluidic paper-based analytical devices with different functions,different metal oxide nanomaterials and artificial antibody technology,a variety of sensing platforms were constructed for sensitive detection of pesticides,miRNA and glycoproteins.The main research work is as follows:?1?A photochemical sensors based on titanium dioxide nanomaterials with good photoelectric properties and artificial antibody technology were constructed for detecting pesticides.Molecule imprinted polymers were initially studied,and their good specificity improved the selectivity of the sensor.The prepared titanium dioxide nanomaterials were characterized and proved that the titanium dioxide nanomaterials with large specific surface area and high electron transport rate have good photoelectric properties.The pesticide chlorpyrifos was selected as the target.Electrochemical method was used to prove that the constructed sensor has a good linear range and a low detection limit,which can achieve sensitive detection of pesticides.?2?An electrochemical sensor based on boronate adsorption molecularly imprinted polymer was constructed.The sensor has a"sandwich"structure:electrochemical tag-target?glycoprotein?-molecularly imprinted polymer.The molecularly imprinted polymer based on boronate adsorption was synthesized on a paper chip modified with Au nanorods,and the selectivity of the sensor was improved by specific recognition of glycoprotein.A cerium oxide nanomaterial with catalytic function for 1-naphthol is introduced,and a silica-gold composite material and a hybridization chain reaction are prepared to prepare an electrochemical signal tag,thereby the electrochemical signal was amplified.?3?Cerium oxide nanomaterials were prepared for constructing paper-based electrochemical sensors.Au nanorods with a large specific surface area and excellent conductivity were grow on paper by in-situ growth.The DNA double-stranded absorped amount of[Ru?NH₃?₆]³⁺,enhancing electron transport capability and achieving electrochemical signal amplification.The multifunctional microfluidic paper-based analytical devices is designed by combining the electrochemical detection and colorimetric detection for achieving rapid and sensitive detection of miRNA.