Determination Of Uranium And Fructose 1,6-Diphosphate Based On Tetradentate Schiff-Base Ligand Emission Spectrometry

Uranium is actinide transition metal elements and has many isotopes.As an important nuclear fuel,uranium plays an important role in national defense and nuclear energy development and utilization.The average content of uranium in the earth's crust is about 2.5 parts per million.Although the content is much higher than Hg,Bi and Ag,it is generally believed that uranium is a rare metal.Uranium is radioactive and chemical toxic,which is very harmful to environmental safety and human health.In fact,uranium that is harmful to the environment mainly comes from uranium mines and nuclear energy development.Therefore,the analysis and detection of uranium in the environment is of great practical significance.Fructose 1,6-diphosphate is an important enzyme regulator?related to carbohydrate metabolism?and an intermediate product of glycolysis.It plays a very important role in organisms by regulating the activity of enzymes related to carbohydrate metabolism.Fructose 1,6-diphosphate has many important effects on organisms,such as protecting myocardium,detoxifying heart,treating brain injury and antagonizing lung injury.It is widely used to treat various metabolic diseases such as coronary heart disease,acute myocardial infarction,renal failure and other diseases.Therefore,the detection of fructose1,6-diphosphate has great application value for disease diagnosis,pharmacological research and drug detection.In view of the importance of uranium and fructose 1,6-diphosphate detection,in this work,we synthesized tetradentate ligand?salophen?and di-tetradentate macrocyclic ligand?H₄L?Schiff-base compounds,and the structure and spectroscopic properties were investigated.Using the ligands to establish a solid phase surface fluorescence sensing method based on Eu?III?-salophen-U?VI?bimetallic system to detect uranium and detect the fructose1,6-diphosphate based on the binuclear uranyl complex?BUH₄L?resonance light scattering method,respectively.These methods provided a new idea for the analysis and detection of uranium and fructose1,6-diphosphate.In the first chapter,the characteristics and research progress of current analytical methods for uranium and fructose 1,6-diphosphate were introduced.The solid phase fluorescence method and resonance light scattering method used in this research and their applications in analysis and detection were introduced.In addition,this paper introduced the research progress of Schiff-base,its application in analysis and the principle of cation-cation interaction.In the second chapter,we synthesized the tetradentate Schiff-base ligand?salophen?and modified it to the surface of the slide through the Si-O bond,and studied its spectroscopic properties.In this experiment,we constructed the Eu?III?-salophene-U?VI?bimetallic system,and established a new method of solid phase surface fluorescence sensing to detect uranium by using the phenomenon that uranyl ions and some cations are close to each other can change its luminescence properties.The results showed that the linear range of uranium concentration detection was 0.02-5 nmol/mL,and the detection limit was 0.008nmol/mL.This method had been applied to the detection of uranium in actual environmental water samples with a recovery rate of 99.5-103.5%.In the third chapter,a di-tetradentate macrocyclic ligand?H₄L?was synthesized in this experiment.Based on the coordination properties of uranyl ion and its strong affinity with nitrogen and phosphorus atoms,a supramolecular polymer system was formed by self-assembly of binuclear uranyl complex BUH₄L and fructose 1,6-diphosphate,and a resonance light scattering method for the determination of fructose1,6-diphosphate was established.Under certain conditions,the resonance light scattering intensity of the supramolecular polymer system had a linear relationship with the concentration of fructose 1,6-diphosphate.Under the optimal conditions,the linear range of this method was 0.4-5nmol/mL,and the detection limit was 0.16 nmol/mL.The content of fructose 1,6-diphosphate in actual samples was analyzed,and the recovery rate was 98.0-102.5%.