A Label-free Immunosensor For Detection Of Nuclear Matrix Protein-22 Based On Chrysanthemum-like Co-MOFs/CuAu NWs Nanocomposite

In this study,a new,simple,and label-free electrochemical immunosensor was presented for the detection of nuclear matrix protein-22?NMP-22?.In order to accurately monitor very small amounts of NMP-22,it was advantageous to use highly efficient nanomaterials as signals.For this reason,we synthesized a chrysanthemum-like nanocomposite?Co-MOFs/CuAu NWs?,using Co-based metal-organic frameworks?Co-MOFs?as carriers and copper gold nanowires?CuAu NWs?wrapped around its surface,which were applied for modifying a glassy carbon electrode?GCE?.The Co-MOFs/CuAu NWs possessed outstanding catalytic capabilities,which served as the signal materials and simultaneously carried the anti-NMP-22 antibody?Ab?.When different concentrations of the NMP-22 antigen?Ag?were specifically attached to the immunosensor,the current responses decreased to varying degrees.The designed biosensor was used the principle to establish a linear regression equation and achieve accurate quantification of NMP-22.After optimization,the NMP-22 sensor exhibited a good linear response over a concentration range from 0.1pg·mL^-1 to 1 ng·mL^-1,with a lower detection limit of 33 fg·mL^-1?based on S/N=3?.The proposed biosensor demonstrated the advantages of ultra-sensitivity,high specificity and acceptable reproducibility,suggesting that the proposed strategy has potential for the quantification of NMP-22 in human urine samples.Moreover,the novel nanocomposite Co-MOFs/CuAu NWs are promising materials for electrochemical sensors to detect other biomolecules.