Preparation Of Multicomponent Boronic Polymers For Bioapplications

As one of the greatest inventions in the history of human beings,polymeric materials have played a significant role in the human society since 1920 s.Thus,polymer science has become one of the most important branches of organic chemistry since then.Recently,the designation,synthesis and preparation of new multifunctionally polymeric materials have become the hottest topic in the field of polymer science.Due to their unique properties as modular reaction,multi-component reactions(MCRs)have gradually attracted the interest of polymer chemists.This type of reaction can generate multi-functional compounds by a one-step reaction,connecting or generating different functional groups,thus constructing novel and unique structures.Therefore,using multi-component reactions as a coupling tool to connect multifunctional groups simultaneously to synthesize multifunctional polymers with complex structures has become a new research direction in the field of polymer chemistry.To verify this concept,this thesis combined the multi-component Hantzsch reaction with the reversible addition-fragmentation chain transfer polymerization(RAFT),using a one-pot method to design and synthesize a fluorescent visible boronic acid containing polymer dihydropyridine structure.Through series of explorations and experiments,controllable and precise preparation of this type of polymer has been achieved.In order to verify the binding ability of this type of boronic acid polymer to monosaccharides,we designed and prepared a new boronic acid with dihydropyridine structure and tested the binding ability of monosaccharides with it.The results showed that the boronic acid with dihydropyridines is similar to the traditional boronic acid in the aspect of binding ability with monosaccharides.In vitro experiments have shown that this type of boronic acid polymer with dihydropyridine structure can reversibly interact with glycoproteins on the cell membrane,thereby protecting cells.In the presence of lectins,cells still had a good dispensability.Therefore,the multifunctional polymer can be potentially used as a visible anticoagulant for cell separation.At the same time,do uble staining experiments and flow cytometry experiments showed th at the polymer had good cell safety property and biocompatibility.In this paper,Hantzsch reaction was taken as a great example to combine multi-component reaction with RAFT polymerization to prepare a visual cell anticoagulant with excellent performance.We believe that this study fully demonstrated the great potential of multi-component reactions in the field of functional polymer synthesis and promoted the application of multi-component reactions in this field.