Development Of Portable And Sensitive Immunoassay Chromatography Test Strip Reader

In recent years the morbidity and mortality of the gastric carcinoma has been stayed at a high level.According to the statistics,the number of new case in our country has been about 42% of the worldwide.The cure rate of the late stage gastric carcinoma is less than 10%,which has been a severe threat to human health.But the cure rate of the early stage gastric carcinoma reaches up to 85%.According to the research,the early diagnosis of the gastric carcinoma can be realized by detecting the concentration of urea enzyme in the helicobacter pylori,leading to reducing the mortality of the gastric carcinoma.Lateral-flow immunochromatographic assay(LFIA)is a simple and efficient method for early detection.It has rapid detection rates,cost-effective and stable results features.Hence,it has now been widely used in drug residue,biomarker of disease and environment contamination.Now many groups has been started in research of the new nanometer materials and the matched detecting devices,which would make the immunochromatographic technology from the simple qualitative detecting to become a quantitative and sensitive method.This article has been made an improvement and optimization of our group's test strip reader.In this paper,a new kind of test-strip reader was designed based on complementary-metal-oxide-semiconductor transistor(CMOS)and tablet personal computers(PCs).This reader is composed of the hardware system and software system.The hardware system is made up of image capture module?barcode scanner module?light source module?power supply module and image processing module.We packaged all the modules and realize the integration of the reader.The functions of the software system include the input of the patient's information?image capture and detection?save and load of the data.We used different denoising methods according to different types of the test strips.We also chose different methods to realize the quantitative detection of the strip.To evaluate the stability and accuracy of this device,different concentrations of urea enzyme samples were tested.The results were very well.This test strip reader has a light weight and can run for long durations without an AC power supply.The detection limit of the urea enzyme samples reached 5 mIU.Above all,this reader can be used for long time clinical test?community examination and outdoor detection.