Synthesis Of Rutin Magnetic Micell Molecular Imprinted Recognition Performance Study

Rutin is a natural active ingredient with anti-oxidative activity,anti-inflammatory and anti-radiation biomedical value,so the separation and extraction of rutin has important research significance.The traditional extraction and separation method will destroy the structure and activity of rutin molecule,which makes the extraction rate of rutin low;and the molecular imprinting technique can be used for separation and extraction due to its high selectivity and predetermined structure.With the development of molecular imprinting technology,Gradually realize the separation and extraction of natural products from weak polar solvents to strong polar solvents.To achieve the molecular imprinting technique for the separation and extraction of rutin from methanol in a strong polar solvent,it is necessary to consider the strong polar solvent to functional monomers and imprinted substances.The weakening of the hydrogen bond between.Therefore,this paper designs and prepares a highly adsorbed material with stable structure and good chemical properties.The polymer can specifically separate the rutin molecule from the medicinal material,and the adsorption mechanism of the polymer is deeply studied through the related experiments and the construction of the adsorption model.The results are as follows:Synthesis of rutin magnetic micelle molecular imprinted material.Two functional monomers identifying the optimal rutin were screened by equimolar variation,and the optimal molar binding ratio was determined?template:4-vinylbenzeneboronic acid:4-vinylpyridine was 1:6:2?.The Fe₃O₄ carrier was synthesized by hydrothermal method and negatively charged by trisodium citrate modification.Pre-polymerization of the best functional monomer and template,incorporating into the micelle molecule under hydrophobic action,selecting a fat-soluble cross-linking agent and a water-soluble cross-linking agent,and a fat-soluble initiator and a water-soluble initiator,and applying the template under ultraviolet light.Cross-polymerization of bifunctional monomer with prepolymer in positively charged micelles,preparation of rutin micelle molecular imprinted material,and then combined with Fe₃O₄ nanoparticle carrier to optimize the amphiphilic molecule,template and crosslinker molar The ratio of 1:0.5:1 was successfully prepared for the molecular beam of rutin magnetic micelles.The structural characterization and properties of rutin magnetic micelle molecular imprinted material were studied.The synthesis of the magnetic nanoparticle carrier was confirmed by scanning electron microscopy?SEM?,X-ray diffraction?XRD?,saturation magnetization curve measurement?VSM?,and infrared?FT-IR?.The SEM,FT-IR and DLS were used to characterize Fe₃O₄@RMMIPs.It was found that at least a part of the micelle molecularly modified Fe3O4?Fe3O4@CA?particles were adsorbed on the surface,which confirmed the successful synthesis of Fe₃O₄@RMMIPs.By measuring the adsorption kinetics of Fe₃O₄@RMMIPs and fitting the four adsorption kinetic models,it is concluded that the secondary model of adsorption kinetics has the best fitting effect,so the adsorbent and adsorbate are realized by chemical adsorption.The adsorption isotherm model has a better fitting curve with the fitted Freundlich model,indicating that the adsorption process of rutin by rutin Fe₃O₄@RMMIPs is monolayer adsorption.Highly specific adsorption of rutin molecules from a mixed solution of three structural analogs of rutin?quercetin,naringin and cyanidin-3-O-glucoside?using a magnetic micelle molecular imprinter The recognition specific factor is as high as 3.55,indicating that the material can separate pure rutin from the complex rutin structural analog mixture.To explore the application of rutin magnetic micelle molecular imprinted material in actual samples.Fe₃O₄@RMMIPs separated rutin from the crude extract of sassafras and showed better selective adsorption capacity,which indicated that Fe₃O₄@RMMIPs can be used as a specific adsorbent and has practical application value.