| Mycobacterium tuberculosis(Mtb),the cause of tuberculosis(TB),is a serious global infectious pathogen with an incidence of up to 10.4 million and a mortality of 1.4 million.Thence,there is great clinical significance of the accurate detection of Mtb for TB's diagnosis and treatment.In this study,we introduced graphdiyne(GDY),a new two-dimensional carbon material,and developed few-layered GDY nanosheets(NSs)with a thickness of only 0.9 nm through a lithium intercalation method which is used as a novel fluorescent sensing platform for the real-time DNA detection.This sensing platform can be used for DNA detection of M.tuberculosis and the identification of its drug resistant mutants with low background and high signal-to-noise rate,and a linear relationship between fluorescence intensity and the concentrations of Mtb was obtained in the range from 0 to 5 nM and a detection limit as low as 25 pM is reached on the condition of acceptable stability and reproducibility.Most importantly,this detection platform provides a potential basis for the development of sensitive,rapid and accurate substitutes for molecular diagnostics of TB. |
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