Construction Of NIR-responsive Cancer Membrane-cloaked Tumor Self-targeting And Carrier-free Nanosystems

Cell membrane-camouflaged nanoparticles for cancer therapy have received a burgeoning interest over the past years.However,the low drug loading and intratumoral release efficiency,and lack of precise targeting remains big challenges;in addition,most drugs were loaded by foreign carriers in the existing studies,which may pose an expected burden in the course of metabolism.In this study,we designed and fabricated a novel NIR-responsive highly targeted carrier-free nanosystem?DOX NPs@ICG@CCCM?by coating the exactly identical source of cracked cancer cell membranes?CCCMs?specifically derived from the homologous tumors onto the surface of the co-assembly nanoparticles of doxorubicin?DOX?and FDA-approved photothermal agent,indocyanine green?ICG?,termed as DIC₃NPs.The nanosystems exhibited a high drug loading capacity?89.8%?,cancer cell self-recognized ability and immune escape function.Further,the nanosystems could be efficiently released for the membrane disturbance triggered by photothermal effect of ICG under NIR irradiation.Meanwhile,the results proved the successful fabrication of the carrier-free nanosystems with high drug loading capacity and strong self-targeting identification ability to homologous cells.The tumor-bearing mice model demonstrated that the self-carried DIC₃NPs nanoparticles possessed a strong intratumoral agminated ability and synergistic chemo-/photothermal therapeutic efficacy against tumors in vivo.The present strategy could be developed as a universal approach for designing and constructing carrier-free theranostic nanovehicles by intentionally selecting specific cancer cell membrane and the inner loading cargoes.