Wheat Breeding And Quality Detection For High-gluten Whole Wheat Flour Using MAGIC Population

Compared with white flour,whole wheat flour has richer nutrients.With strengthening of healthy diet concept,whole wheat flour now accounts for a higher proportion of food raw materials,but there have been litter study on wheat breeding research and food processing quality testing for whole wheat flour,and the system of cultivation and evaluation is incomplete.The quality of the gluten content of whole wheat flour directly determines the food processing use,most of wheat for high-gluten whole wheat flour depends on imports.Moreover,the whole wheat flour retains the outer structure such as bran,which leads to serious pesticide residues problem.In this paper,four genetically interrelated multi-parent advanced generation inter-cross(MAGIC)were constructed using four wheat varieties in the middle and lower reaches of the Yangtze River as parents,and the yield and disease resistance of each strain in the population were studied.The processing quality of the grain and the prepared whole wheat flour were identified and statistically analyzed,and the related genetics were analyzed for developping the corresponding molecular marker-assisted breeding.As a result,the purpose of this study was to acquire the preferred wheat varieties used for the high-gluten whole wheat flour production,for promoting the high-gluten whole wheat flour and reducing the problem of pesticide residues from the root causes.The main research work and results are as follows:(1)Screening suitable materials to create a wheat MAGIC population.The wheat cultivar Zhenmai 168,Yangmai 16,Yangmai 20 and Yangmai 22 were used as parents to construct a MAGIC population containing 332 independent stable lines.What's more,the yield and the resistance of wheat powdery mildew of each strain were statistically analyzed.(2)The whole wheat flour processing quality was tested and the preferred wheat lines suitable for producing high-gluten whole wheat flour were selected.The water content,protein content,hardness,1000-grain weight,wet gluten content,stiffness coefficient,dilution value,SDS sedimentation value,liquefaction value and solvent retention of the seeds of the parents and MAGIC populations were determined and statistically analyzed,respectively.Lines 167 is the preferred lines for the production of high-gluten whole wheat flour.(3)The genetics research was carried out and a molecular marker-assisted breeding system was optimized.An efficient SSR marker library used to locate the PmV resistance gene for powdery mildew resistance was constructed;the powdery mildew resistance genes Pm21 and PmV were finely mapped and the corresponding molecular markers were developed for tracking.The hardness-related genes Pina and Pinb were cloned and the Pinb gene type was identified in the MAGIC population.Lines 167 contains the powdery mildew resistance gene PmV,and its Pinb gene type is Pinb-D1 b.