Superhydrophobic Surface Modification And Performance Evaluation Of Anti-Adhesion Membrane For Postoperative Repair

Anti-adhesion membrane is a medical product used to prevent postoperative adhesion.It can isolate the injured part from adjacent tissues and play an antiadhesion role.Electrospun nanofiber membranes have attracted extensive attention in the field of anti-adhesion due to their light and flexible texture and good mechanical properties.They are ideal anti-adhesion structural materials,but there are also problems such as limited anti-adhesion effect of nanofiber membrane.Enhancing the anti-adhesion effect of medical membranes and developing a suitable and effective anti-adhesion product are still hot and difficult points in this field.The antibiotic adhesion effects of superhydrophobic surfaces have already been reported by many researchers.By introducing superhydrophobic anti-biological adhesion properties,superhydrophobic modification of anti-adhesion membrane can further enhance its anti-adhesion function.Therefore,this subject used electrostatic spray technology to prepare superhydrophobic microspheres.The microspheres were sprayed on the PCL/PLGA electrospun fiber membrane to improve the anti-blocking effect of the fiber membrane,and then a superhydrophobic anti-adhesion composite membrane was obtained.The surface properties and anti-blocking effect of anti-adhesion composite film were further characterized and evaluated.,which provided a certain research basis for product development.In this project,electrostatic spraying technology was used to conduct electro spray by hydrophobic SiO₂/ PCL/ PLGA blend solution.Different electrospray microsphere coatings were prepared by changing the content of SiO₂.The morphology and hydrophobicity of the microspheres were characterized by SEM and contact angle test.The results show that electrospray method can construct multi-scale microspheres to form rough surface.And with the increase of the proportion of SiO₂,the hydrophobic property of the coating surface was enhanced.When the content of SiO₂ is more than 50%,the contact angle on the surface of the electrospray coating can be greater than 150°,forming a superhydrophobic surface.Meanwhile,superhydrophobic composite membranes was prepared by electrospraying a superhydrophobic microsphere coating on PCL/PLGA nanofiber membrane.The effect of electrospray time on the surface properties of the composite membrane was investigated.The composite membrane was characterized by SEM,FTIR,EDS and contact angle tests.The results indicate that with the increase of spraying time,the microspheres on the surface of the fiber membrane increase evenly,the contact angle increases gradually.When the electrospray time is about 2 hours,a stable superhydrophobic layer is formed on the surface.The superhydrophobic composite film's contact angle can reach more than 150°,while rolling angle is less than 10°,has good self-cleaning performance,.The composite film with 100% SiO₂ content of microspheres in the electrospray layer can maintain stable superhydrophobic properties immersion in PBS solution for 7 days.Finally,the superhydrophobic composite membrane was tested for cytotoxicity,protein adsorption and cell adhesion.The results show that the composite membrane has good biocompatibility.Moreover,compared with PCL/PLGA fiber membrane,the superhydrophobic composite membrane with 100% silicon dioxide content in the electrosprayed microspheres had obvious effect of preventing protein and cell adhesion.In summary,by electrospraying a superhydrophobic coating on the nanofiber membrane,this paper successfully prepared a super-hydrophobic composite membrane which can be used as an anti-adhesion membrane for postoperative repair.And the superhydrophobic composite membrane with 100% silica content of the microspheres have good biocompatibility and anti-protein cell adhesion effect.This research results have important reference value for the development and preparation of a new generation of anti-adhesion products.