Studied The Preparation Of Water-soluble Fullerene Drug Carriers And Their Performance In Drug Release And Bioimaging

As one of the most important science and technology in twenty-first Century,nanoscience has played an important role in material preparation,electronic computer,medical health,aeronautics and Astronautics,environment,energy and biotechnology field.The preparation of nanoparticles with fullerene which has unique propertie has been widely studied by biomedical researchers.Gadolinium based fullerene nanoparticles show great potential in the field of biological imaging.The water soluble fluorescent nanoparticles C60-TEGs was prepared by self assembly of fullerenes and three contraction four glycol.The fullerene C60 was modified into fluorescent nanoparticles by combining the hydrophilic group of three ethylene glycol four glycol,which greatly improved the water solubility of fullerenes and obtained good fluorescence imaging properties.The morphology and particle size of nanoparticles were characterized by transmission electron microscopy(TEM)and dynamic light scattering(DLS).Docetaxel was loaded on fullerene C60-TEGs nanoparticles by thin film dispersion.The encapsulation efficiency of nano drug delivery system DTX/C60-TEGs is high,with a minimum of 75.5% and a maximum of92.7%.Drug release follows the mechanism of drug surface analysis and diffusion.After 24 h release,the release curve of drug release begins to become relatively slow,and the drug molecules release slowly by overcoming the pion pion accumulation of fullerenes.DTX/C60-TEGs was co cultured with human hepatoma cell HepG-2,and the bioimaging and cytotoxicity of DTX/C60-TEGs in vitro were evaluated.The results showed that DTX could effectively inhibit the proliferation of tumor cells after the release of the nanoparticles on the surface of the nanoparticles.After 48 h culture,the inhibitory rate of the cells could reach 81.7%,84.5%,89.4%and 91.2%,respectively.LSCM images showed that the signal of C60-TEGs of fullerene nanoparticles could be detected clearly in HepG-2 cells,which could clearly reflect the growth state of cells.Water soluble gadolinium fullerenes Gd@C82-TEGs nanoparticles were prepared by self assembly method.The morphology and particle size of the nanoparticles were characterized by transmission electron microscopy(TEM)and dynamic light scattering(DLS).By cytotoxicity test,human breast cancer cells were cultured with gadolinium-based fullerene nanoparticles with a concentration of 12.5 mg/L to 100 mg/L.The cell morphology of gadolinium-based fullerenes at different concentrations was observed by electron microscopy.It is found that gadolinium based fullerene nanoparticle solution has no obvious effect on cell metabolism and structure,and the survival rate of cells is at least 92%.Which shows the gadolinium based fullerene nanoparticles has no toxicity to cells.The T1 relaxation time and MRI effect of gadolinium based fullereneswith different concentrations were observed by low field nuclear magnetic resonance imaging system.The relaxation efficiency of gadolinium based fullerene nanoparticles is about 1.5.It indicate a good relaxation efficiency.T1 weighted imaging shows that gadolinium based fullerene nanoparticles with excess of 0.1 mmol/L can exhibit strong magnetic resonance signals.