Gel Fiber Preparation Based On Laminar Flow And 3D Printing Technology

Fibers have many advantages as carriers,and the fibers are generally relatively slender and easy to bend,even in the case of folded crimps,without damaging their structure.Using these material properties,the fibers can be interwoven and bundled to form a more complex fiber network.These complex fiber network structures can be used for three-dimensional cell culture in vitro or for the construction of more complex three-dimensional tissues in vitro.It is well known that many tissue structure of organisms are layered and fibrous.For this reason,fiber materials have received great attention as a carrier in the fields of constructing bionic blood vessels and tissue engineering.Fibers prepared based on microfluidic technology have been used to simulate tubular,multilayer tubular or other fibrous structures.3D printing is an emerging technology that is widely used in the preparation of tubular structures,providing a new platform for constructing complex bionic tissues.However,in the preparation of hydrogels with microchannels loaded cells,3D printing technology generally requires precise stacking and separate preparation of individual layers,and the bio-ink required for printing is very expensive.Therefore,it is necessary to further improve the preparation method of the gel fiber.In this study,different devices were designed by two techniques and used to prepare gel fibers of different sizes,which further improved the preparation method of gel fibers,and provided a new idea for constructing multi-layer vascular tissue in vitro.The results obtained by this study are as follows:1.A microfluidic chip device was designed and fabricated,and a series of adjustments were made to optimize the composition of each phase fluid,the flow rate,and the fluid concentration.The exploration of the laminar flow rate was completed,which provided key parameters for the subsequent experiments on the preparation of gel fibers.2.Based on microfluidic laminar flow technology,a capillary kit device was designed and fabricated.By adjusting the flow rate and concentration of each phase fluid,various sizes of gel microfibers from 100 ?m to 1000 ?m and hollow gel microfibers were successfully prepared,further perfected the controllability of microfluidic laminar flow technology for gel fibers.3.Based on 3D printing technology,a series of reusable molds are provided,which are simple in design,do not need for pro-hydrophobic modification,and are short in production time.The multi-layer hollow composite gel fiber was successfully prepared by the method of multiple nesting and peeling of the mold.