Fabrication Of Mesoporous Silica Hybrid Nanoparticles For Anti-Tumor Therapy

Malignant tumors threaten the health and life of human.Traditional treatment methods are usually accompanied by side effects,relapse,and poor patient compliance and other shortcomings.The combination of multiple treatments for tumor has become the trend of clinical tumor therapy.Combined therapy can make up for the deficiency of single therapy and achieve better therapeutic effect.In addition,with the development of biotechnology,protein drugs with pharmacological activities have appeared.Compared with traditional chemotherapy drugs,protein drugs have clear biological function,high activity and strong specificity.The delivery of bioactive protein drugs into tissues or cells directly solves clinical problems and becomes an efficient treatment.With the development of nanotechnology,nanomaterials are more widely applied in the field of biomedicine,which brings new hope for the diagnosis and treatment of tumors.Among them,mesoporous silica nanoparticles have the characteristics of adjustable size,easily modified surface and mesoporous pore diameter,which can be used to load chemotherapy drugs.However,how to construct effective nano-drug delivery system and realize controlled drug release,how to construct multifunctional nano-drug delivery system and realize the combined treatment of tumor are still very important issue in tumor treatment.In view of the above problems,this paper studied the application of two drug delivery systems in tumor treatment,and the main research contents are given below:?1?Based on the limitation of protein drugs in clinical tumor treatment,we present a pH-responsive nanocomposite for protein delivery and cancer therapy.Large pore size mesoporous silica nanoparticles?LPMSNs?were obtained by adjusting the templating agent during synthesis.In view of the lysosome environment,the acid-sensitive amide bonds were modified on the surface to obtain a charge-conversional LPMSN triggered by pH values?LPMSN-DMMA?.In order to prevent the leakage of protein drugs,gold nanoparticles?AuNPs?were adsorbed on the surface of LPMSNs as gatekeepers through electrostatic attraction.When exposed to acidic lysosome microenvironment,surface charge of LPMSNs converted from negative to positive due to the breakage of2,3-dimethylmaleamic acid groups.Thereafter,the adsorbed AuNPs disassociated from the surface of LPMSNs because of the electrostatic repulsion,resulting in controlled release of CC and further initiating the apoptosis machinery of cancer cells.This study provides a promising platform for encapsulation,delivery and controlled release of proteins.?2?A multifunctional composite nanocarrier was designed for the delivery of DOX and cancer therapy.Mesoporous silicon-coated gold nanorod nanomaterials?AuNR@mSiO₂?combine the photothermal effect of gold nanorods with the drug loading capacity of mesoporous structure.The copper sulfide nanomaterials?Cu_xS NPs?were assembled with AuNR@mSiO₂ by electrostatic adsorption,and the biocompatibility of the materials was improved by surface modification of PEG.The composite nanocarrier can generate active oxygen under the near-infrared laser irradiation,and can increase the local temperature of the tumor.At the same time,the release of DOX brings the effect of chemotherapy.It has achieved the integration of photothermal therapy,photodynamic therapy and chemotherapy,and provided a new idea for the material design of combined therapy for tumor.