Biological Cell Morphological Characteristics And Fluorescence Spectroscopy Technology

Liver cancer is a malignant tumor that seriously threatens human health.However,the mortality rate can be effectively reduced with the early screening of liver cancer.The early symptoms of liver cancer are not obvious,and the related diagnoses are also mostly detected by liver tissue,which is costly and causes great pain to the patients.Sufficient understanding of liver cells and liver disease cells is the key to achieve early detection of liver cancer.Therefore,the combination of fluorescence spectroscopy and cell morphology study to detect and analyze liver cells and liver disease cells can provide a spectral basis for early screening of hepatic carcinoma.Firstly,spectroscopic studies were carried out to obtain the emission spectra of hepatic cell HL-7702 [L-02],hepatic fibrosis cell LX-2 and hepatic carcinoma cell HepG-2 at five cell concentrations by spectroscopy experiments.And the Gauss Multi-peak Fitting Technology was used to analyze each peak center,peak height,and peak width of the emission spectrum.In addition,morphological studies were performed on the three cells,and the differences in cell diameters were analyzed by fluorescence microscopy and flow cytometry.Secondly,use nonlinear fitting curve to judge the relation between cell diameter and fluorescence saturation intensity.Finally,combine with relevance judgment,the correlation between the maximum fluorescence intensity and the cell diameter was analyzed.Experimental results showed that there were two specific fluorescence peaks in hepatic cell HL-7702 [L-02],hepatic fibrosis cell LX-2 and hepatic carcinoma cell HepG-2 between 550 and 750 nm.However,hepatic fibrosis cell and hepatoma carcinoma cell also have a third specific fluorescence peak at 726 nm.The average diameters of normal hepatic cell HL-7702,hepatic fibrosis cell LX-2,and hepatic carcinoma cell HepG-2 were approximately 13.1 ?m,16.6 ?m,and 17.2 ?m,respectively.When the cell concentration increases,the maximum fluorescence intensity was saturated more distinctly with the increasing cell diameter.There is a strong correlation between the cell diameter and the maximum fluorescence intensity.In this thesis,the combination of fluorescence spectroscopy and morphological studies was adopted to judge the influence of cell morphology differences on fluorescence spectrum,which can more accurately compare liver cells and liver disease cells,accordingly,laying the foundation for exploring accurate and efficient diagnosis of liver cancer.