| Problems such as thrombosis and restenosis have always been a difficult problem to be solved in interventional therapy.The biocompatibility of the material can be enhanced by surface modification of the scaffold material.Metal Organic Framework?MOF?is a new type of coordination material assembled from metal junctions and organic ligands.It has broad prospects in the fields of catalysis and drug delivery.Copper ions in copper-based MOF are more capable of catalyzing the release of nitric oxide?NO?from endogenous nitric oxide donors,but this material has rarely been reported for surface modification of cardiovascular materials.The HKUST-1 coating?M10,M20,M30?was prepared by layer by layer method?LBL?on the surface of alkali-activated titanium.The preparation of HKUST-1coating was confirmed by XRD,XPS,FTIR and SEM analysis.The sample was folded at a large angle,and the SEM results confirmed that the coating had good mechanical stability.The NO release test showed that the NO release rate was proportional to the number of deposited layers.The rate of NO release from normal endothelial cells ranged from?0.5-4?×10-10 mol·cm-2·min-1,and the rate of NO released by HKUST-1 coating?M10?deposited on 10 layers was?5.0±1.5?.×10-10mol·cm-2·min-1.M10 was immersed in a reaction solution containing EDTA?500?M?,PBS solution containing albumin?BSA 2 mg/ml?,and cell culture medium?FBS15%?for 2 h,and then subjected to NO catalysis test.The samples all released NO stably,and the release rate was within the range of NO release rate of normal endothelial cells.The results of platelet adhesion test showed that M10 had obvious inhibition of platelet adhesion and activation.The results of half-body blood circulation test showed that M10 had excellent antithrombotic properties.The above experiments proved that M10 has good blood compatibility.Endothelial cells?EC?,smooth muscle cells?SMC?and macrophage?MA?cells were statically cultured on the surface of M10 coating.The results showed that M10 could promote EC and inhibit the adhesion and proliferation of SMC and MA cells.The use of hydrogen peroxide?200?M?to damage EC mimics the oxidative stress state,and the results demonstrate that M10samples can alleviate endothelial cell peroxidation damage caused by hydrogen peroxide.The M10 coated titanium wire was implanted into the rat abdominal aorta,and the results showed that the coating had a good inhibitory effect on intimal hyperplasia.Based on the above research,three layers of dopamine?DM?were deposited on the surface of various base PVC,316L and Ti-O materials as the connecting layer,and the M10 coating was successfully prepared by LBL method.The coating can stably release NO and inhibit platelet activation,further broadening the application range of HKUST-1 coating.In summary,the layer self-assembly method can qualitatively regulate the copper content on the surface of the sample,which can regulate the NO release rate.In vivo and in vitro evaluation results show that the HKUST-1 coating combines the NO function of copper ion catalyzed by copper ions and the physiological action of copper ion itself,synergistically achieving inhibition of platelet adhesion and activation,anti-smooth muscle proliferation,anti-inflammatory and promoting endothelialization Features. |
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