| The issue of public food safety is related to the the stabilization of society and the safety of the people.It has aroused much attention recently,since the food safety problems occur frequently.Along with social economy development and the lives of the people level enhancement,animal food have more and more extensively appeard in people's daily life,at the same time,its quality and safety have also received increasing attention all over the word.One of the most striking thing for food security is the residues of veterinary drugs in foods owing to the misapplication and illegal use of veterinary medicine,which resultes in potential harmfulness to health of human beings.The residues of veterinary drugs in food should be aroused great attention,and the effective determination method is the guarantee for food safety.However,due to the complicated matrix of food samples and low levels of veterinary drugs to be tested,it is necessary to conduct the processes of preconcentration and separation prior to instrument analysis.So,it is essential to create a simple,efficient and green environment-friendly method for detection.MSPE technology,first proposed by safarkov and safarkhas evolved into one of the most promising methods for sample pretreatment.It is based on the use of a magnetic sorbent,which is dispersed into the liquid sample matrices enabling the target analyte adsorbed on the surface of the adsorbent,then seperated by an external magnetic field,and eventually eluted by a suitable solvent.Nowadays,MSPE technology has been widely applied to the analysis of food,environmental and biological samples owing to its advantages such as simplicity of operation,rapidity and being solvent-free.For the purposes of this article,the approach based on magnetic solid-phase extraction combined with high liquid chromatography was presented for the microextraction and determination of the veterinary drug residues in food.The concrete research content is as follows:1.A detection method using graphene oxide-loaded magnetic nanocomposite based extraction technique coupled to HPLC was developed for the determination of phenol in the honey sample.Some major parameters having impact on extraction efficiency were investigated and optimized.Under the optimal conditions,the proposed method displayed a good linear range(0.05–125?g·L-1),with coefficients of determination?R2?of 0.9986.The limits of detection?S/N=3?was 0.02?g·L-1.The recovery was 86.0%–92.7%and the relative standard deviation was 3.1%(c=0.1?g·L-1,n=7).The results showed that the method was successfully employed to determine the level of phenol in the honey sample.2.A detection method using polystyrene-loaded magnetic nanocomposite based extraction technique coupled to HPLC was developed for the determination of malachite green and crystal violet in the drinking water sample.Some major parameters having impact on extraction efficiency were investigated and optimized.Under the optimal conditions,the proposed method displayed a good linear range(0.1–100?g·L-1).The limits of detection?S/N=3?of malachite green and crystal violet were 0.03?g·L-1and 0.01?g·L-1,respectively.The recovery was between 84.0%–90.0%and the relative standard deviation was between3.49%–5.68%.The results showed that the method was successfully employed to determine the level of malachite green and crystal violet in the drinking water sample.3.A detection method using chitosan-loaded magnetic nanocomposite based extraction technique coupled to HPLC was developed for the determination of three tetracyclines in the milk sample.Some major parameters having impact on extraction efficiency were investigated and optimized.Under the optimal conditions,the chlortetracycline and oxytetracycline displayed a good linear range(1–100?g·L-1)and the tetracycline was in the range of 5–100?g·L-1.The limits of detection?S/N=3?of chlortetracycline,oxytetracycline and tetracycline were 0.12?g·L-1,0.15?g·L-1and 0.5?g·L-1,respectively.The recovery was between 85.6%–90.7%and the relative standard deviation was between 2.16%–5.49%.The results showed that the method was successfully employed to determine the level of three tetracyclines in the milk sample. |
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