The Construction Of Composite Nanoparticles For Protein Detection

Proteins are of tremendous importance in biological studies and clinical diagnosis.Recently,wide attention has been attracted to the detection of protein by homogeneous assays,which with sensitivity and simple operation.In this paper,combined with polymer-assisted signal amplification,nanotechnology and protein homogeneous detection technology,a composite nanoparticle sensing system based on competitive identification was designed.1.The macro chain transfer reagent with aryl sulfonamide ends was synthesised by click chemistry and coupled reaction,and the polymer SABA-PEG4000-b-PAAn-SH with electronegative polyacrylic acid fragments was prepared by reversible addition-fragmentation chain transfer(RAFT)polymerization.At the same time,the homopolymer HS-PAAAn with reducing was also synthesised by RAFT polymerization.The characterizations showed D<1.4,indicating the polymerization activity was controllable.The ends modified with the thiol group provided the possibility the synthesised of polymer-modified gold nanoparticles.2.Dendritic mesoporous silica nanospheres(MSNs)with good size distribution and excellent load performance were prepared by biphase stratification approach,and Cu2+was loaded in MSNs.The aminated mesoporous silica nanospheres(NH2-MSNS)were prepared by amination experiments.The polymers modified the gold nanoparticles by Au-S coordination to form polymers-grafted gold nanoparticles.The negatively charged polymers-grafted gold nanoparticles are adsorbed on the surface of the NH2-MSNS by electrostatic interaction,and achieved the effective encapsulation of Cu2+.The characterizations showed the construction of the composite nanoparticle detection system.