Functionalization And Anticancer Applications Of Silicon-based Mesoporous Materials

Silicon-based mesoporous materials have large specific surface area,abundant pore structure and reaction sites.Silicon-based mesoporous materials not only can chelat,adsorb and load proteins,drugs,heavy metal ions,etc.,but also can use surface reaction sites for functional modification.At present,silicon-based mesoporous materials have been widely used in catalysis,sensing element,adsorption,biomedicine and other fields.Silicon-based mesoporous materials can be divided into natural silicon-based mesoporous materials and synthetic silicon based mesoporous materials according to their sources and preparation methods.Compared with natural silicon-based materials,synthetic silicon-based materials have adjustable morphology and pore size.In this paper,functional silicon-based mesoporous materials obtained by functional modification were used to chemo-photothermal therapy and gene therapy.The main content of this paper can be divided into the following two aspects:1.To study the chemo-photothermal therapy of silicon-based mesoporous materials,we designed and prepared a kind of core-shell structure nanoparticle with copper selenide core and mesoporous silica shell.Then,the ability of controlling drug release and cancer treatment effect were studied in detail.Oil-soluble copper selenide(Cu2-xSe)nanoparticles with high conversion efficiency were synthesised by high temperature injection method,and then mesoporous silica coated on the surface of Cu2-xSe by ligand exchange.Then,we used lauric acid to encapsulate drug(TPDOX)in the mesoporous silica,named Cu2-xSe@MSNs-TPDOX.The experiment results showed that Cu2-xSe@MSNs-TPDOX had high photothermal conversion efficiency,good photothermal stability and excellent ability of controlled drugs release.In the biological part of this chapter,we used human breast cancer resistant strains(MCF-7/ADR)and drug-resistant strains(MCF-7).The results showed that Cu2-xSe@MSNs-TPDOX can overcome multi-drug resistance both in vivo and in vitro.Compared with chemotherapy and photothermal therapy only,Cu2-xSe@MSNs-TPDOX can effectively kill cancer cells by chemo-photothermal therapy.2.To study the application of silicon-based mesoporous materials in the field of gene therapy,we designed and prepared a kind of organic and inorganic hybrid gene carrier according to N,N-dimethylamino-ethylester(PDMAEMA)grafted diatomite,named diatomite gene carrier.The excellent biocompatibility of diatomite and the excellent gene carrying capacity of PDMAEMA were combined to produce a gene carrier with excellent performance,simple synthesis and low cost.Diatomite was used as initiator and polymerization was carried out.Gel electrophoresis experiment showed that the diatomite gene carrier could load DNA effectively.Gene delivery experiment showed that DNA can be delivered into cells by diatomite gene carrier.