Development Of Fumonisin B₁ And T-2 Toxin Fluorescent Immunochromatographic Test Strips

Four visual fluorescence immunochromatographic assays for rapid detection of fumonisin B1(FB1)and T-2 toxin were described in this paper,Which can be used for rapid detection of two mycotoxins in com,rice,millet and barley samples.In the development of quantum-dot-labeled immunochromatographic test strip for fumonisin B1(FB1-QDICA),the activated ester method was used to prepare quantum dots-antibody(QDs-Ab)conjugates and the optimal working condition was deter mined,the optimum coupling molar ratio of quantum dots,antibody and activators was 1:2:1500,and pH 7.4 PBS as test strip buffer,and the dilution of goat anti mice IgG and coating antigen(FB1-OVA)were 400 and 40 respectively,and the dosage of QDs-Ab and working buffer were 0.3 ?L and 5 ?L.Under optimal conditions,the FB1-QDICA exhibited visual limit detection 2 ?g/L and 20 ?g/kg in samples.In the development of fluorescence quenching immunochromatographic test strip for FB1(FB1-FQICA),the quantum dots labeled OVA(QDs-OVA)and the colloidal gold labeled anti-tetracycline antibody(AuNPs-Ab)was prepared,and the optimal working condition was deter mined:the optimal amount of K2CO3(0.2 mol/L)and antibody added to prepare gold labeled antibody were 20 ?L and 25 ?L,and pH 7.4 PBS as test strip buffer,and the dilution of the FB1-OVA and QDs-OVA were 1:10 and 20,and the dosage of AuNPs-Ab was 3 ?L.Under optimal conditions,the FB1-FQICA exhibited visual limit detection 1 ?g/L and 10 ?g/kg in samples.The optimal working condition T?2-FQICA was deter mined:the amount of K2CO3(0.2 mol/L)and antibody added to prepare gold labeled antibody were 10 and 50 ?L,and pH 7.4 PBS as test strip buffer,and the dilution of the T-2-BSA and QDs-OVA were 1:12 and 20,and the dosage of AuNPs-Ab was 3 ?L.Under optimal conditions,the T-2-FQICA exhibited visual limit detection 2 ?g/L and 20 ?g/kg in samples.The FB1 and T-2 multi residue FQICA exhibited visual limit detection 1 ?g/L and 2 ?g/L respectively and 10 ?g/kg and 20 ?g/kg in samples.The test time was 15 minutes.The test strip detection method had good specificity by cross-reaction,and the results agreed well with a commercial enzyme linked-immunosorbent assay kits.