Study On Fluorescence Immunoassay For The Detection Of Artazine

In this paper,up-conversion fluorescent nanomaterials and fluorescent microspheres were selected as fluorescent label materials.UCNPs-based fluorescence immunoassay with magnetic separation and FMs-based immunochromatography were established for rapidly quantitative detection of atraine in foods respectively.OA-UCNPs was prepared by thermal decomposition method.The ligand exchange method was used to prepared water-soluble upconversion nanoparticles.Atrazine antibody conjugated to upconversion nanomaterials form fluorescent signal probe by activated ester method,coated antigens conjugated to magnetic polystyrene microspheres form capture probe.After optimization of conditions,when the amount of signal probe was 70 ?L,the amount of capture probe was 50 ?L,and the incubation time was 50 min,UCNPs-based fluorescence immunoassay with magnetic separation was established for the detection of atraine.the detection limit of atrazine was 0.005 ?g L-1,the linear range from 0.005 to 10 ng mL-1,the immunoassay showed the similar recognize capacity for prometiyn and propazine in comparison with atrazine,and can be used to detect ATR?prometryn and propazine at the same time.The water samples and sugar cane juice samples can be directly analyzed without any pretreatment by this fluorescence immunoassay.Corn and rice samples need to be simply extracted,and then the sample extract is diluted 5 times with PBS(pH 7.4)and detected.The recoveries of atrazine ranged from 84.68%to 119.33%in the spiked samples.And the results of the developed fluorescence immunoassay exhibited good correlation with the analysis results of LC-MS/MS(R2=0.9927).Atrazine coated antigen(Atrzzine-OVA)conjugated to carboxyl functionalized fluorescent microspheres to form fluorescent probe by activated ester method.The concentration of the C-line and T-line coating materials of the test strip,the type of the sample diluent,the type of the nitrocellulose membrane,and the addition amount of the fluorescent probe and the working fluid were optimized,then,under optimal conditions,FMs-based immunochromatography were established for quantitative detection of atraine.The quantitative detection limit of atrazine was 0.01 ng mL-1.The occurrence of cross-reaction can be caused by prometryn and propazine and can be used to detect ATR?prometryn and propazine at the same time.The water and sugar cane juice samples were diluted 5-fold and 15-fold with PB(pH 6.0)to detect,and corn and rice samples need to be simply extracted,and then the sample extract is diluted 20-fold with PB(pH6.0)and detected.The recoveries of atrazine ranged from 83.27%to 115.5%in the spiked samples.And the results of the developed fluorescence immunoassay exhibited good correlation with the analysis results of LC-MS/MS(R2=0.9912).