Research On Manufacturing Of Tissue Engineering Scaffold Based On Electrospinning Technology

The tissue engineering scaffold is a three-dimensional porous carrier required for cell growth.The scaffold must have many properties such as good biodegradability,biocompatibility,mechanical properties,and non-toxicity.In terms of bionics,the structure of most human organs and tissues is similar to nanofibers,so nanofibers can be used as scaffold materials for organ and tissue repair.Using electrospinning technology can produce a three-dimensional porous fiber membrane,under certain circumstances,to replace the three-dimensional tissue structure for replication.The structure of this fiber scaffold is exactly the same as that of the natural extracellular matrix,and the specific surface area and porosity are relatively high,this allows cells to migrate,adhere and proliferate,allowing cells to differentiate and grow in different directions.Chitosan has good biocompatibility,is non-toxic and biodegradable,and is widely used in tissue engineering materials.However,there are few studies on the mechanical properties of chitosan nanofibers in the current research on changing the material ratio and electrospinning process,so improving the mechanical properties of the material itself by changing the process parameters has certain application reference value.The rich functional groups on the surface of graphene oxide can interact with chitosan through chemical bonds,which can improve the structural stability and interface strength of the scaffold.At present,there are relatively few studies on the material system of the combination of chitosan and graphene oxide,therefore,giving full play to the comprehensive performance of the chitosan graphene oxide composite system is of great significance to the research and application of electrospinning technology in tissue engineering scaffolds.This subject is based on the electrospinning method,using chitosan as the base material,adding PEO,PVA,GO and other components,starting from the perspective of optimizing the material system and preparation process to improve the mechanical properties,surface morphology and biological activity of the material itself,and give full play to the comprehensive performance of the composite system.Through systematic analysis and characterization of its structural morphology,mechanical properties and biological effects,the feasibility of electrospinning chitosan nanofibers as tissue repair materials was confirmed.The main research contents and results are as follows:1.Design orthogonal experiments to optimize the preparation process conditions of CS / PEO nanofiber membranes,the best conditions for obtaining electrospun CS / PEO nanofiber membranes are: CS: PEO mass ratio 8: 2,voltage 16 V,receiving distance 10 cm.The CS / PEO nanofiber membrane prepared under this condition has a relatively thin fiber diameter,a relatively uniform and smooth surface,a tensile strength of 14.2 MPa,and an elongation at break of 43.6%.2.Prepare different ratios of CS / PEO / PVA nanofibers.When CS: PVA is 1: 9,the maximum elongation at break of CS / PEO / PVA nanofiber membranes reaches 30.2%,and the tensile strength slightly decreases to 20.4 MPa.The diameter and porosity of nanofibers increase and decrease with the increase of PVA content.The surface of CS / PEO / PVA nanofibers is flat and smooth,with uniform pores,which is conducive to cell adhesion and proliferation.3.The addition of a small amount of GO can increase the tensile strength of the CS / PEO / PVA / GO composite nanofibers.The nanofibers of CS / PEO / PVA / GO are smooth and uniform.As the GO content increases,the diameter of the nanofibers becomes smaller and the thickness is uneven.When the GO content is 0.2%,the finest fiber diameter can reach about 280 nm.4.The ordered CS / PEO / PVA / GO nanofibers are prepared by the method of magnetic field assisted electrospinning,and the nanofibers with very good order degree are collected,which has more application value for ordered tissue engineering scaffolds.When the GO content is 0.05%,the order of the nanofibers is the best.5.The biological properties of the CS / PEO / PVA / GO nanofiber scaffolds were tested by in vitro cell culture experiments and mouse liver cells(NCTC1469)were seeded on the nanofiber membranes.The experimental group of CS / PEO / PVA / GO nanofibrous scaffold material was significantly increased,indicating that the cell compatibility of CS / PEO / PVA / GO fibrous scaffold is very good,which is helpful for cell adhesion and proliferation,and demonstrates the feasibility and superiority of the prepared nanofibers in tissue engineering scaffold materials.