| Electrochemical and fluorescence detection technologies have been applied in various fields due to their convenient operation,less consumption and rapid response.They are very popular in various research fields such as environmental monitoring and drug analysis.In this paper,the two excellent analytical methods were used to study.Magnetic electrochemical sensors based on imprinted polymers were designed to specifically detect acetaminophen?AP?,and fluorescent sensors based on surfactant were designed to detect uranyl.The details are as follows:The first chapter.This chapter mainly reported the research status of AP,the wide application of electrochemical methods,the development and application of molecular imprinting technology,and the classification and application of electrochemical sensors based on imprinting technology,then reported the application of Fe3O4 magnetic nanoparticles in molecular imprinting,the application of computational chemistry in imprinting technology,then described the research background and significance of uranium,and the classification of surfactants and their application in fluorescence detection technology.The second chapter.A sensor based on magnetic surface molecularly imprinted membrane?MMIP?was prepared for high sensitivity and high selectivity determination of acetaminophen?AP?.Firstly,the appropriate functional monomers and solvents required for the polymer were screened,and the molecular electrostatic potentials?MEP?were calculated by the DFT/B3LYP/6-31+G in computational chemistry.As imprinting materials prepared by bifunctional monomers or multifunctional monomers are more stable and more specific than polymers prepared by monofunctional monomers,bifunctional monomers methacrylic acid?MAA?and 4-vinyl pyridine?4-VP?were finally selected according to the calculated chemical results and actual requirements.MMIP with high recognition performance was synthesized based on magnetic nanoparticles Fe3O4@SiO2 with excellent core-shell structure.Next,the magnetic electrode?MCPE?was constructed by filling neodymium-iron-boron magnet into carbon paste electrode?CPE?.MMIP/MCPE sensor was prepared by attaching imprinted polymer to the electrode surface under strong magnetic field.The performance of this sensor was detected by differential pulse method?DPV?.The sensor exhibited a linear dependence on the concentration of AP from 6×10-88 to 5×10-55 mol L-1and 5×10-55 to 2×10-44 mol L-1,with a detection limit based on the lower linear range of 1.73×10-88 mol L-1?S/N=3?.The results showed that the recovery rate of the sensor in actual samples was 95.80103.76%and RSD was0.78%3.05%.The third chapter:A fluorescence sensor with simple operation,low price and high sensitivity was prepared to detect uranium.Firstly,the Salophen ligand was synthesized,and it could be quickly combined with UO22+to form a stable Uranyl-Salophen complex due to its special cavity structure.Then,the complex was coated with sodium dodecyl sulfate?SDS?surfactant,which achieving the effect of sensitizing fluorescence intensity and more intuitively and conveniently to detect uranium.The reaction time,temperature,SDS dosage and pH conditions of the system were optimized respectively.Under the optimal conditions,the fluorescence intensity showed a good linear relationship between 0.0053.5 mol/mL of Uranyl concentration,and the recovery rate of Uranyl detected in actual samples was 96%103%. |
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