Effect Of Organicphosphorus Flame Retardant On Microalgae And Its Fatty Acid Composition

With the ' development of petrochemical industry,a large number of industrial products,especially polymer products,not only bring convenience to people,but also bring safety risks and unknown pollution.With the improvement of analytical technology and the strengthening of environmental awareness,various pollutants produced by it have been gradually paid attention to and studied.The fire caused by the flammability of polymer materials often causes great loss of life and property and environmental pollution.The production of flame retardants alleviates this situation to some extent,but it also makes flame retardants become a common pollutant.As the ocean is an important "reservoir" of various pollutants,organophosphate flame retardants have been found in all major sea areas and even in polar regions far from human activities.Among them,tris(2-chloroethyl)phosphate is one of the main organophosphorus flame retardants detected in the Marine environment.Previous researches on organophosphorus flame retardants were mainly conducted in the fields of production,performance optimization and cost control.In recent years,researches on organophosphorus flame retardants mainly focused on environmental investigation,while researches on the impact of organophosphorus flame retardants on organisms were less.In order to better understand the effects of organic phosphorus flame retardants for marine organisms,based on the laboratory culture,two kinds of marine microalgae(Nitzschia closterium and Platymonas helgolandica var.tsingtaoensis)as test organisms.The growth monitoring indexes and the composition of algal cell fatty acids of microalgae exposed to tri(2-chloroethyl phosphate)were measured,and the effects of tri(2-chloroethyl phosphate)on the growth of two kinds of micrbalgae and the changes of algal cell fatty acid composition were discussed.The main conclusions are as follows:exposure to tris(2-chloroethyl)phosphate at different concentrations can stimulate the growth of Nitzschia closterium and Platymonas helgolandica var.tsingtaoensis in different degrees,but the stimulation effect is gradually weakened in the later stage,and prolonged exposure to tris(2-chloroethyl)phosphate can cause weak growth inhibition of Nitzschia closterium.The decrease in stable isotope ratio also indicated that prolonged exposure to higher concentrations of tris(2-chloroethyl)phosphate may reduce microalgal cell viability.The composition and content of fatty acids of different microalgae species were significantly different.16:0,16:ln-7 and 20:5n-3 were the dominant fatty acids of Nitzschia closterium,while 18:3n-3,16:0 and 20:5n-3 were the main fatty acids of Platymonas helgolandica var.tsingtaoensis.Treatment with tris(2-chloroethyl)phosphate at a concentration of 1 mg/L will affect the composition of microalgal fatty acids in the later stages and enhance the accumulation of polyunsaturated fatty acids.Tris(2-chloroethyl)phosphate is not acutely toxic to microalgae,and short-term exposure to tris(2-chloroethyl)phosphate will not cause significant cell damage in microalgae,but its weak Toxic effects will manifest after prolonged exposure of pollutants.This paper is the first to use marine microalgae to study the effect of organophosphorus flame retardant tris(2-chloroethyl)phosphate on organisms.At the same time introduce stable isotope analysis technology and fatty acid analysis into the effect of organophosphorus flame retardants on organism.The long-term exposure potential toxicity of organophosphorus flame retardants was discussed,which provided a basis for future research on organophosphorus flame retardants and the response mechanism of microalgae to environmental stress.Although the current concentrations of organophosphorus flame retardants in the environment do not have a significant toxic effect on microalgae,the long-term effects of organophosphorus flame retardants in the context of increasing pollutant inputs still need to be vigilant.