Multi-Protective Effects Of Wheat Embryo Globulin On D-gal-induced Aging Mice

Wheat germ is rich in functional nutrients,which the protein content is as high as 30%.The proteins in wheat germ mainly include water-soluble protein(albumin),salt-soluble protein(globulin),alcohol-soluble protein(gliadin),and alkali-soluble protein(glutenin).Wheat embryo globulin is a common protein with a variety of physiological activities,such as anti-inflammatory activity,antioxidant activity,and immunity enhancement.It is an important part of wheat germ protein,accounting for about 20% of wheat embryo globulin.At present,there is no theoretical support for related researches on the in vivo antioxidant activity of wheat germin and the effect on the intestinal flora of aging organisms.Early analysis of wheat embryo globulin through proteomics and bioinformatics revealed that wheat embryo globulin may be involved in the metabolic pathway of Alzheimer disease.Based on this,the multiprotective effects of wheat embryo globulin on D-gal-induced aging mice were studied.(1)Investigate the improvement of WEG on cognitive dysfunction in aging miceD-galactose was used to induce aging mice,wheat embryo globulin and huperzine A were used to intervene in aging mice.The analysis showed that the injection of D-gal resulted in rapid body weight gain,decreased activity,dull coat color,and even shedding;after intervention with wheat embryo globulin,the activity of the mouse increased,the body weight increased slowly,and the coat color recovered,with obvious improvement.It can be seen from the organ index that D-gal causes abnormal enlargement of the spleen and liver of mice,and the immune imbalance.Wheat embryo globulin can relieve organ swelling and regulate immunity.Using new object recognition and water labyrinth experiments to study the spatial learning and memory function of mice,it was found that wheat embryo globulin can improve the discrimination index of new objects in mice(P<0.01),reduce the escape latency of mice(P<0.05)Increase the platform quadrant percentage(P<0.05),as well as increase the residence time of mice in the original platform quadrant(P<0.05)and the number of crossing the original platform position(P<0.01).Using histopathology to analyze the brain tissue,it can be concluded that wheat embryo globulin has a certain recovery effect on brain cells,and at the same time reduces the damage of D-gal to neurons in the hippocampus of mice.(2)Analysis of the protective effect of WEG on oxidative damage in aging mice.After the behavioral experiment was completed,blood was taken through the eyeballs,the mice were dissected to remove various organs,serum was prepared,and brain tissue,liver tissue,and intestinal tissue were prepared into 10% tissue homogenate.The antioxidant indexes of mouse serum,brain tissue,liver tissue,and intestinal tissue were detected respectively,and it was found that wheat embryo globulin can significantly increase the T-AOC,GSH-Px and SOD activity significantly reduces the content of MDA in serum and brain tissue,but has no significant effect on MDA in liver and intestinal tissue.(3)Investigate the effect of WEG on the intestinal flora of aging mice.Through ?11umina technical analysis fecal intestinal flora in mice,using RDP classifier bayesian algorithm on the 97% similarity level OTU representative sequence taxonomical analysis.A total of 1 domain,1 kingdom,47 phylum,91 classes,215 orders,381 families,755 genera,1193 species and 1958 OTU units were involved.According to the Alpha diversity analysis,there was little difference between the indexes in the model group and the blank group,and the Coverage indexes were above 99.80%.Wheat embryo globulin could decrease Shannon index(P<0.01)and increase Simpson index(P<0.01).At the phylum level,the dominant intestinal microorganisms in each group were Firmicutes,Bacteroidetes,Actinobacteria,Proteobacteria,Patescibacteria,and Tenericutes.At the genus level,The dominant genera of intestinal microorganisms in each group were Lactobacillus,norank_f_Muribaculaceae,unclassified_f_Ruminococcaceae,Enterorhabdus,Desulfovbrio,Candidatus_Saccharimonas,Steptococcus,Lachnospiraceae_NK4A136_group,unclassi-fied-_f_Lach-noSpiraceae,Bacteroides,norank_f_Lachnospiraceae,Lachnoclostridium,Enterococcus,Blautia.By comparing the values of Bacteroidetes / Firmicutes(B/F)in the four groups,it was found that: in the aging model group,B/F significantly decreased(P<0.01).After the intervention with wheat embryo globulin and huperzine A,the intestinal flora of B/F in the aging mice was worth improvement,with a certain rising trend.Wheat embryo globulin can lower the relative abundance of Patescibacteria(P < 0.05),the relative abundance of Epsilonbacteraeota and Tenericutes(P<0.01).Then improve intestinal flora disorders,delay the aging process.Wheat embryo globulin can effectively improve cognitive dysfunction of aging mice,protect the ability of oxidative damage in aging mice,and increase the relative abundance of beneficial bacteria in the intestine of aging mice.Wheat embryo globulin can be used as a type of special medical food to prevent aging.