| According to the characteristics of high proportion of industrial wastewater in the water inflow of a sewage treatment plant in wujin district,changzhou city,jiangsu province,and high requirement of effluent water quality,this paper investigated the water quantity and water quality of upstream enterprises,and analyzed the characteristics of pollutants discharged by upstream enterprises.According to the water quality characteristics and the upstream enterprise waterworks A2 O process operation,set up the A2 O + denitrification filter pilot plant,plant simulation run,choose has special effects on features of pollutant degradation ability of embedding bacterium,to biological strengthening of A2 O process,selection of COD,ammonia nitrogen,total nitrogen,total phosphorus of conventional pollutants,aniline as the characteristics of pollutants,investigates the A2 O microorganism of the pilot scale system,the effect on pollutant removal by adjusting the feed water flow,research HRT for system for pollutant removal efficiency;The effect of internal reflux ratio on removal of ammonia nitrogen and total nitrogen was studied by adjusting the reflux ratio of nitration liquid.Microbial composition was analyzed by high-throughput sequencing.Denitrification bacteria attached to the surface of the filter material were strengthened by adding high-quality carbon source to the denitrification filter to improve the denitrification effect of the system.Microbial community characteristics under different carbon sources were analyzed by high-throughput sequencing.The total phosphorus in effluent was reduced by adding a dephosphorization agent.(1)through the research on the upstream enterprise industrial wastewater quantity,the sewage treatment plant water from industrial waste water is higher,accounting for about 40%of the sewage treatment plant water inflow,of which printing and dyeing wastewater,electronic electroplating wastewater,mechanical processing waste water is given priority to,the normal operation of the high proportion of industrial wastewater of sewage treatment plant will inevitably produce certain effect.According to the analysis of the conventional water quality sampling of the upstream enterprises,many of the conventional indicators of the upstream enterprises have the problem of exceeding the standard,and the characteristics of the conventional indicators of the wastewater discharged into the pipe network of the enterprises conform to the characteristics of the enterprises,such as the high COD of the wastewater discharged by the printing and dyeing enterprises.The content of Cu,Fe and Zn in wastewater treatment plants and water samples before and after pretreatment was determined by ICP-AES.In particular,the raw water of the electronics factory contains a large number of copper ions,and the wastewater of the coating factory contains a large number of zinc ions.Gel chromatography,3d fluorescence and gc-ms were used to analyze the organic compoundsin wastewater treatment plants and water samples before and after pretreatment on behalf of enterprises.The results showed that all the water samples had different levels of toxicity,all of which were at the level of poisoning or above.If a large number of water samples entered the water plant,the normal life activities of microorganisms in the activated sludge system would be greatly affected.Aerobic biodegradability experiments were carried out on the water inlet of sewage treatment plants and water samples before and after pretreatment on behalf of enterprises.The aerobic biodegradability of wastewater in different enterprises was different.Although COD was removed to a certain extent,it did not fall to the ideal level.(2)through the biological enhancement of the pilot system of A2 O,the research shows that the COD removal rate is 57%,ammonia nitrogen removal rate is 89%,total nitrogen removal rate is 44%,total phosphorus removal rate is 62%,aniline removal rate is 41%.Overall,the removal rate is not ideal.With the addition of aerobic and anaerobic bacteria,the removal of COD,total nitrogen,total phosphorus and aniline by pilot trial A2 O increased.With the addition of aerobic bacteria,the removal of COD improved from 53.7% to 71%.Ammonia nitrogen removal rate reached 91%,total nitrogen removal rate reached 59.8%,aniline removal rate reached 64.1%.(3)high-throughput sequencing was carried out on the water plant and the pilot test system.From the level of the gate,Proteobacteria,Chloroflexi,Actinobacteria,Bacteroidetes,and Acidobacteria were relatively abundant in all samples,and Proteobacteria occupied an absolute advantage.At the level of subordination,the dominant genus mainly includes norank-fnorank-o-1-20,norank-f-caldilineaceae,Hyphomicrobium,norank-f-norank-o-dadabact eriales,Defluviicoccus,etc.(4)different carbon sources were added to the denitrification filter to study the effect of the ratio of carbon to nitrogen on the denitrification filter,and the following conclusions were obtained: when sodium acetate was used as the carbon source,the removal rate of nitrate in the denitrification filter reached the highest of 95.4%,the total nitrogen removal rate reached86.93%,and the effluent COD did not increase significantly.With methanol as the carbon source,when C/N is 4~5,the denitrification filter has the highest removal rate of nitrate up to96.3%,the total nitrogen removal rate reaches 86.86%,and the effluent COD does not increase significantly.In the case of sufficient carbon source,the denitrification filter with sodium acetate as the carbon source has no accumulation of nitrite along the reverse direction,and nitrate degradation is fast.In the denitrification filter with methanol as the carbon source,nitrite accumulation occurred along the direction,and nitrate degradation was slow.(5)high-throughput sequencing of the two filters showed that Proteobacteria was the main phylum,Chloroflexi and Actinobacteria were the secondary phylum in the two filters.The relative abundance of denitrification related bacteria in the denitrification filter withsodium acetate as the carbon source was 20.67%,21.92%,33.02% and 37.41% from the sampling port,respectively.For the denitrification filter with methanol as the carbon source,the relative abundance of denitrification related bacteria in the sampling port was 9.71%,16.65%,12.38% and 8.51%,respectively.(6)the removal effects of PAC,PFS and PASF on total phosphorus in secondary sedimentation tank and the removal effects of PASF,PAM and magnetic powder combination on total phosphorus were compared and optimized by orthogonal test.The following conclusions were obtained: the removal rate of total phosphorus when PSAF was added alone was higher than PAC and PFS.In terms of the removal effect of total phosphorus,PSAF+PAM>PSAF+ magnetic powder +PAM>PASF+ magnetic powder >PASF was obtained when PSAF+PAM>PSAF+ magnetic powder +PAM>PASF+magnetic powder.According to the orthogonal experiment results,when PASF dosage is 80mg/L and PAM dosage is 0.4mg/L,the total phosphorus removal rate is 89.51%. |
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