Study On The Preparation And Properties Of Organosilicon Polymer Fluorescent Materials

Due to the diversity of the group structure of organosilicone polymer,its structure and application are rich.Functional groups with different properties are connected to polysiloxane materials by bonding,which increases the variety of organosilicone polymer products and broadens the application range.Rare earth elements are introduced into the polysiloxane matrix to prepare rare earth polymer photoluminescent materials.Polysiloxane materials can not only coordinate well with certain rare earth ions,but also efficiently absorb energy from the excitation light.It is transferred to rare earth ions to enhance the luminescent properties of the rare earth ions,thereby improving the luminescent properties of the organosilicon-rare earth ion complex,and thus enhancing its application value in biological therapy.Compared with single photon,two-photon has the fluorescence characteristic of deep penetration,so it is very urgent for two-photon absorbing materials in various application fields.Therefore,the preparation of a kind of polysiloxane self-repairing elastomer with two-photon absorption characteristics is of great significance for its value enhancement in biological applications.An organosilicon fluorescent probe was prepared by combining naphthalimide dye with polysiloxane polymer.It is also applied in biological imaging to lay a foundation for the reversible detection of organosilicon polymer fluorescent probe.This paper from the following three aspects,this paper designs and synthesizes polysiloxane fluorescent materials with different luminescent properties through simple thiol-ene click reaction,Mannich reaction and amide reaction,and then studies the related properties of the materials.The application value of polysiloxane fluorescent materials in biological imaging has been explored.I.A series of novel functionalized silicone polymers P1 and P2 were designed and synthesized based on thiol-ene click reaction.The polymer has excellent thermal stability,photorefractive index and low cytotoxicity.After that,a series of organosilicone polymer with unique fluorescent properties were obtained by coordinating the functionalized organosilicone polymer with rare earth ion Eu³⁺/Tb³⁺.The complexes containing Eu³⁺showed typical pink fluorescence emission,and the complexes containing Tb³⁺also showed typical green fluorescence emission.The fluorescent intensity increased with increasing rare-earth coordination ion ratio.This fluorescent material has been successfully applied to bioimaging,and its intracellular fluorescent emission could correspond to fluorescence spectrum in vitro,showing its potential application value in biological diagnosis.II.A series of self-healing organosilicone elastomers Cns with two-photon absorption properties were designed and synthesized based on Mannich reaction.The elastomer has simple synthesis method,excellent optical properties,thermal stability,self-healing properties and excellent mechanical properties.Both the solution of polymer D1 and the elastomer Cns showed blue atypical luminescence.Most importantly,we could detect the unique two-photon emission in the elastomer.Due to the stability and low toxicity of organosilicon elastomer,cell culture experiments were carried out.The experimental results showed that the elastomer Cns could conduct cell culture well,indicating its potential application in biological imaging.This work provides a new research idea for the atypical two-photon luminescence of organosilicon self-healing materials.III.A polysiloxane fluorescent probe T1 based on naphthalimide dye was designed and synthesized based on an amide reaction for reversible determination of hypochlorous acid/glutathione.The polysiloxane fluorescent probe had high sensitivity,stability and cell permeability.The intrinsic solution of T1 emitted typical green fluorescence,and when hypochlorous acid was added to the solution of T1,the fluorescence intensity varied widely and almost all were quenched.When glutathione solution was added,the fluorescence intensity was restored to 80%.T1 was applied to cell imaging in vivo based on its obvious color changes.We found that T1 not only had reversible hypochlorous acid/glutathione detection in cell imaging,but also had excellent two-photon properties.In addition,probe T1could also achieve zebrafish hypochlorous acid/glutathione reversible detection in vivo.The polysiloxane fluorescent probe has potential applications in distinguishing the relationship between the oxidation of hypochlorous acid and the reduction of glutathione.