The Fabrication And Application Of Electrochemical Sensor Basing On The Molybdenum Selenide Composite Nanomaterial

Cancer is a serious and incurable disease that threatens human health and life.The studies have shown that early diagnosis can effectively reduce the mortality of cancer patients.As of now,using effective analytical methods to detect tumor markers closely related to the incidence of cancer is the focus and direction of researchers.Electrochemical immunosensor analysis has become a promising method for detection due to its advantages of fast and accurate analysis and convenient preparation.In this study,molybdenum selenide composite nanomaterial with excellent electrochemical performance was designed and prepared for electrochemical immunosensing analysis.The main contents are as follows:?1?Sandwich-type electrochemical immunosensor based on Au@Pt DNRs/NH₂-MoSe₂NSs nanocomposite for the sensitive detection of alpha-fetoproteinA composite?Au@Pt DNRs/NH₂-MoSe₂ NSs?of Au@Pt dendritic nanorods loaded by amino functionalized molybdenum selenide nanosheets was designed and synthesized.A sensitive sandwich-type electrochemical immunosensor was fabricated for the quantitative detection of AFP by using Au@Pt DNRs/NH₂-MoSe₂ NSs as the label of secondary antibodies?Ab₂?.The Au@Pt DNRs/NH₂-MoSe₂ NSs not only exhibited better catalytic activity towards the reduction of hydrogen peroxide?H₂O₂?,but also offered rich anchors for bioconjugation.Meanwhile,gold nanoparticles anchored on amino functionalized graphene composite?Au NPs/NH₂-GS?with admirable conductivity and biocompatibilityw was synthesized and chosen to be the matrix material to improve detection sensitivity.Under optimal conditions,the measured amperometric current responses exhibited a good linear relationship with the logarithm values of AFP concentration in the range from 10 fg m L^-1 to 200 ng m L^-1 and a detection limit as low as 3.3 fg m L^-1?S/N=3?.Compared with previous literatures of AFP detection,the immunosensor showed favourable repeatability,selectivity and stability.?2?Label-free amperometric immunosensor based on Au@Pt DNPs/MoSe₂@MoS₂ for squamous cell carcinoma antigen detectionUrchin-like gold@platinum dendritic nanoparticles supported on molybdenum selenide@molybdenum disulfide nanocomposite?Au@Pt DNPs/MoSe₂@MoS₂?was successfully synthesized as the matrix platform of label-free amperometric immunosensor for the quantitative detection of squamous cell carcinoma antigen?SCCA?.Au@Pt DNPs/MoSe₂@MoS₂ consists of Au@Pt DNPs with splendid biocompatibility as well as catalysis and MoSe₂@MoS₂ with reformative peroxidase activity,which owned enhanced electrocatalysis toward hydrogen peroxide reduction and steady bioconjugation for bioprotein immobilization.Additionally,Au@Pt DNPs/MoSe₂@MoS₂ possessed satisfactory conductivity to accelerate electron transfer at the electrode interface.With the help of Au@Pt DNPs/MoSe₂@MoS₂,the immunosensor showed a good linear extent(100 fg m L^-1-100 ng m L^-1)for SCCA detection,reached a lower detection limit(33.3 fg m L^-1).