The Synthesis And Application Of Novel Fluorescence Probes Based On FRET Mechanism For Detection Of SO₂ Derivatives

In nature environment,there are several of ions,including cations,anions and small bioactive molecules such as sulfite,reactive oxygen copper ion,iron ion,glutathione,and so on.Most of them could participate in many important biological processes of human beings in different forms,for example,regulating metabolism,immune responses.The level of these ions always keep dynamic balance in living cells so that the organim could keep healthy.Once the balance is broken,it will induce disorders followed by a variety of disease.Thus,it is urge to develop methods for detecting exogenous and endogenous ions in vivo and in vitro.From now on,many traditional methods have been applied to detect microelement such as elerochemistry,titration,chromatography,and flow injection analysis.Compared with traditional way,fluorescence probe is more practical and noticeable because of its overwhelming advantages such as rapidity,high selectivity,and sensitivity,low cost,non-invasive detection,good bio-compatibility as well as real-time deteetability.In recent years,a surging number of fluorescence probes have been developed.In this paper,three kinds of probes for detecting sulfur dioxide(SO2)derivatives based on FRET mechanism were presented.1.A novel ratiometric and mitochondria-targeted fluorescence probe based on pyridine-TCF platform for detecting sulfur dioxide derivatives in living cells.In previous reports,coumarin was used as donor of fluorescence probe due to its high quantum yield and good biocompatibility.TCF group has many good properties including high fluorescence quantum yield,long wavelength emission and good cell permeability.Therefore,TCF is easily designed as the energy acceptor in Forster resonance energy transfer(FRET)-based fluorescent probes.In this work,using couarin-pyridine as energy donor and mitochondria-targeted group,a ratiometric FRET-based fluorescence probe was developed.The low toxicity allowed this probe to be used to detect SO2 derivatives in living cells.Moreover,the low detection limit,good selectivity,good water solubility and mitochondria-targeted provided the potential for the further research and even deeper bio-applications.2.A fluorescence probe based on phenothiazine-hemicyanine platform.According to the previous work of fluorescence probe based on phenothiazine,it is likely that phenothiazine has potential for appling as energy donor.As a result,a fluorescence probe based phenothiazine-hemicyanine platform was developed.The probe shows high selectivity and low detection limit.However,it is a pity that the energy transformation of this probe from donor to acceptor is low,so phenothiazine is unfit for energy acceptor.3.A fluorescence probe based on coumarin-pyridine-quinoline platform.Base on our successful attempt of using coumarin-pyridine as energy donor,probe KPC based on coumarin-pyridine-quinoline platform was designed and synthesized.