Properties And Application Of New Injectable Sodium Hyaluronate/Sodium Alginate Hydrogel

As a three-dimensional network-like porous material,hydrogel material can be loaded with therapeutic elements,such as drugs,cells,and cytokines.Applying hydrogel materials containing therapeutic elements to pathological locations such as tissues and organs not only functions as a bionic scaffold,but also releases the contained therapeutic elements to help tissues and organs recover.This material has gradually been widely used in tissue engineering.However,some hydrogels have the disadvantages of poor biological compatibility and long degradation cycle,which do not meet the requirements of tissue engineering.In view of this,we have prepared a new type of injectable sodium hyaluronate/sodium alginate biohydrogel,on the basis of determining its biological safety,to study its degradation rate and mechanical properties under different conditions.Further,in order to investigate the effect of this type of hydrogel material in practical applications,this thesis further developed two aspects of application research.The details are as follows:(1)By changing the mass content ratio of the two precursor substances of sodium hyaluronate and sodium alginate,1%-5%,2%-4%,3%-3%and 4%-2%were prepared respectively For different hydrogels,investigate the degradation ability and mechanical properties of these gels in vitro.The results showed that within a certain range,the degradation rate and mechanical properties showed a certain correlation.The 3%-3%hydrogel had the most degradation residue in 22 days,which was 43.51%,and the highest compressive strength was 0.122 MPa;while 1%The gel degradation rate of-5%is the fastest,with 13.03%remaining in 22 days,and its compressive strength is also the lowest at 0.021 MPa.This experiment proves the independent selectivity of the performance of the hydrogels we prepared.For different pathological environments,we can choose a suitable hydrogel ratio.(2)Application research of loaded triamcinolone acetonide nano-gel hydrogel in the treatment of rabbit model periodontitis.The combination of triamcinolone acetonide nano-drugs prepared by high gravity technology and hydrogel materials can improve the dispersibility and stability of water-insoluble drugs in the hydrogel matrix,and realize the long-term release of drugs in the periodontitis site.The treatment effect of periodontitis,compared with the existing clinical periodontitis treatment drugs,proved the advantages of hydrogel preparations.(3)Application study of adhesive hydrogel-titanium alloy composite scaffold material loaded with stem cell-derived exosomes in the treatment of rat skull defects.The hydrogel material was modified with cell adhesion peptide(RGD),and the adhesive hydrogel loaded with mesenchymal stem cell exosomes was further filled into the porous titanium alloy to prepare a composite scaffold material for rat skull Repair of defects.Adhesive hydrogels have excellent biocompatibility and can promote the migration and adhesion of osteogenic stem cells in vivo;titanium alloy materials perfectly compensate for the inherent mechanical defects of hydrogels,so that the composite scaffold material can be applied to bearing bone defects Repair;stem cell exosomes can induce and promote osteogenic differentiation of osteoblastic stem cells in the body,and avoid the drawbacks of traditional stem cell therapy.