Bioassays Based On Nano-infinite Coordination Polymers

Disease markers play an important role in the pre and prognostic stages of the disease,and play an extremely important guiding role in the prevention,diagnosis,treatment and post-recovery monitoring of various diseases.In recent years,researchers have been developing rapid,sensitive and efficient detection methods for disease markers.Among the various methods,enzyme linked immunosorbent assay?ELISA?is highly recommended due to its high specificity and sensitivity.However,the traditional ELISA also has many problems,such as the enzyme is easy to deactivate,the construction process is tedious,the single signal output mode,the enzyme load is low and the detection environment requirements are high.The introduction of new functional nanomaterials provides a new way to solve the above problems.Infinite coordinate polymer?ICP?nanoparticles are a kind of new nanomaterials,which are composed of metal bodies and organic bridge ligands.Once discovered aroused the interest of the vast number of researchers.Compared with other members of coordination polymers?such as metal-organic framework materials?,ICP exhibits controllable structure and object adaptive encapsulation capability,easy functionalization and modification,and more importantly,rapid response to external stimuli,enabling it to be widely used in the field of field analysis.1.Infinite coordination polymer nanoparticles?FeICP?with iron ions as the main body were synthesized by photolysis and FeICP has peroxidase-like properties.Therefore,FeICP combined with the secondary antibody to make the nanometer beacon.FeICP nanometer beacon could catalyse H₂O₂ to produce hydroxyl radical?OH·?,and OH·oxidized 3,3',5,5'-tetramethylbenzidine?TMB?to produce the TMB(TMB_OX)of the oxidation state.The solution of the detection system changed from colorless to blue.At the same time,TMBOX has a strong light absorption capacity at 808 nm,and the temperature will significantly increase when the 808 nm laser irradiate the solution after the reaction.A sandwich immunosensor for detection of cTnI was constructed.The dual mode signals could be read only by using plate reader and digital thermometer and realize sensitive detection of cTnI.2.Based on the simple operation,high sensitivity,strong anti-interference and low requirement for the detection of instruments and equipment,a new two-mode accurate and sensitive method for the detection of glutathione?GSH?was developed for colorimetric and photothermal applications.The oxidase properties of FeICP were used to catalyze TMB to become the oxidation state TMB?TMBOX?to appear blue,and TMBOX could redox with glutathione,and the colorimetric strength was inversely proportional to the concentration of GSH.TMBOX has strong light absorption at 808 nm.After a short irradiation with 808 nm laser,the solution heats up rapidly??35?T?,which is inversely proportional to the concentration of GSH.Thus,dual-mode sensitive detection of GSH colorimetric and photothermal was realized.3.FeICP was doped with molybdenum ions with high efficiency by using FeICP's adaptive encapsulation capability,which greatly improved the peroxidase-like properties of FeICP.Mo@Fe ICP nanoparticles were combined with recognition unit secondary antibody?Ab2?to obtain nanoscale beacons?Mo@FeICP-Ab2?,Mo@FeICP-Ab2 to specificity recognition,object under test when the addition of exogenous H₂O₂ and para-phenylene diamine?OPD?,Mo@FeICP could catalyze OPD-H2O2 system produce 2,3-diaminophenosine oxazine?OPDox?and make the solution changed from colorless to yellow.At the same times,OPDox can produce strong fluorescence.Based on the above principles,we successfully designed and developed a new Mo@FeICP enhanced colorimetric and fluorescence dual mode method for detection of prostatic special antigen.