Rejuvenation Preservation And Active Substance Determination Of Termite Balls

Medicinal,edible fungi are increasingly valuing by researchers due to their unique natural activity and medicinal value,especially insect parasitic fungi such as Ophicordyceps Sinensis and Cordyceps militaris,which have proved to be of extremely high medicinal value.Fibularhizoctonia sp.is a symbiotic fungus found in termite nests.Previous s tudies have shown that the mycelium has steady growth and capacity to replicate and high yield.It has excellent growth opportunities in terms of availability.In the present research,we focus on the deterioration and rejuvenation of termite balls,the protection of mycelium and active substances,and conducts quality test indicators and the inherent medicinal edible value of termite balls in cultivation expansion,to provide a basis for the future production of termite balls.The main findings are as follows:(1)Rejuvenation of strains.In order to maintain the fabulous traits of strains,through microscopic inspection of the quality of the strains at different conceivable,the observation indicators and preservation methods of their adequate traits were developed.The hyphae structure is durable,white with lush branches helped in diffusion.It is consider as a mother plant for preservation and passages to controlled by 3 ways.When degeneration occurs,the quality testing and screeningcarried out,strains that maintain the original reproductive ability improved through cutting-edge separation and rejuvenation technology.(2)Mycelium safety.The acute toxicities test showed that male and female rats did not die.The experimental animal anatomy did not re veal any apparent pathologic changes in the organs.Therefore,male and female rats were gavaged through the oral maximum tolerated dose(MTD),suggesting non-toxicity of the mycelial material.(3)Amino acid analysis.The total amino acid content of mycelium reaches 16.35%,including 7 essential amino acids imperative for the human body: Leucine(Leu),Lysine(Lys),Methionine(Met),Threonine(Thr),Phenylalanine(Phe),Valine(Val),Iso-Leucine(Ile),essential amino acids were 30.5% of the total amino acid content.(4)Other active substances.Ergosterol,a natural alcohol substance of fungi,has a content of 3070 ?g/g in mycelium,which is higher than that of Ganoderma lucidum spore powder and Ophicordyceps Sinensis.High content of ergosterol promotes the integrity and membrane binding of mycelium showing enzymatic activity,robust growth and reproduction vigor under wet conditions.The content of cordycepic acid is 33.27 mg/g,which is slightly lower than that of wild-type Ophicordyceps Sinensis,but the cultivation conditions are simple and fast reproductionwith high yield expanding the culture objective to increase the yield of cordycepic acid.(5)Fungal polysaccharides.According to the improved ultrasonic-water extraction and alcohol precipitation method,the crude polysaccharide extraction rate of mycelium powder was 25.05%,extraction rate was 85.60%,and the actual content of fungal polysaccharide in the mycelium powder was 21.40%,which was higher than that of common medicinal fungi.In the in vitro antioxidant test of polysaccharides,it shows that its antioxidant capacity increases with the concentration of polysaccharides,showing good antioxidant activity.Fungal polysaccharides developed as one of the essential fungal active substances.It has high value to become a high-quality polysaccharide product.In summary,termite balls have good growth and reproduction potential and can be applied to deep fermentation.The external morphological characteristics of mycelium and the dynamic changes of ergosterol content can be used as indicators for the quality control of bacterial degradation.To develop potential,more in-depth research should be carried out.