Detection Of Metal Nanoparticles In Biological Tissues By SpICP-MS Method And Investigation Of Lu³⁺ By Schiff Base Fluorescent Probe

Metal nanomaterials are the most widely used nanomaterials.With their extensive application,these metal nanomaterials will inevitably enter the environment,through food,water,air and other ways,and eventually into the human body,creating potential risks to human health.It has been reported that in addition to its own chemical properties,the health toxicological effects caused by metal nanoparticles are related to particle concentration and diameter distribution.Therefore,accurate assessment of its health risks requires accurate quantification of the metal nanoparticles?MNPs?containing particle size,mass concentration and particle number.At present,Current technologies mostly reveal the physical characteristics of nanoparticles in water environments or simple matrices.However,there are few methods and techniques for rapid and accurate detection of concentration and particle size distribution of metal nanoparticles in biological matrix,and a complete detection system is lacking.Single particle inductively coupled plasma mass spectrometry?spICP-MS?is a fast,sensitive?ng/L?detection and analysis technique that can be based on the particle concentration and particle size distribution of nanoparticles,and can also characterize the ionic.A unique and optimized alkaline digestion method was used to release metal nanoparticles from biological matrix.We compared the digestion effect and background of the enzyme digestion method and the alkali digestion solution with different purity from different manufacturers.Finally,the electronic grade purity alkaline digestion solution was selected.The standard particle recovery experiment and ion protein binding verification experiment were designed.The recovery rate of standard gold nanoparticles?AuNPs?was 95.9%,which was in the normal range..Then spICP-MS technology was used to comprehensively detect two common benthic organisms that are easy to be exposed by nanoparticles:clams and oysters.The total concentrations of 40 kinds of metal elements in two kinds of seafood were determined by ICP-MS,and 20 kinds of metal were selected to be analyzed by spICP-MS.The results showed that 5 MNPs?Y,La,Ce,Pr,Gd?were detected in clams.Five MNPs?Y,La,Ce,Pr,Nd?were also detected in oysters.The particle size distribution of MNPs in clams and oysters was between 35-55 nm and 30-65 nm,and the particle concentration and particle size distribution were different among different metals.The possibility of this method is verified by TEM experiment.Based on this analysis,the health risks of metals in both seafood were assessed by comparing the temporary tolerable weekly intake?PTWI?recommended by the world health organization?WHO?/food and agriculture organization?FAO?with the ion concentration limits of Chinese food standards.These results provide important information about the presence of metal nanoparticles in seafood.To our knowledge,this is the first time rare earth element nanoparticles have been found and reported in bivalve mollusk tissues.In the experiment,sp ICP-MS was also used to detect the metal particles in various organs of dogs in lead-zinc mining areas.The results showed that there were Pb metal particles in the lung tissues of dogs.The particle size of Pb metal particles was distributed between 45 nm and 65 nm,and the particle mass ratio was about 5%of the total amount of Pb metal in lung tissues.Some low concentrations of metal particles have been detected in other organs,but further characterization is needed.Exposed metal particles in the air may enter the lungs and other tissues through respiration and blood circulation,thus posing a great potential hazard to biological health.The characterization of the concentration and size characteristics of metal nanoparticles provides more detailed information for their biological health and toxicological effects.Finally,the ion state detection of metal nanoparticles,especially the trace ion detection of rare earth,is also combined with the fluorescent probe technology.The schiff base fluorescent probe has a specific selectivity to Lu³⁺.Fluorescence detection is simpler and faster than icp-ms,and it is also a promising detection method for trace ion detection in the future.