Assembly Of Porphyrin-based Metal-organic Framework For Biosensor Application

Metal-organic frame?MOFs?has become a kind of widely used multifunctional material with simple synthesis,orderly structure,adjustable aperture,diverse shapes,large specific surface area and easy functionalization.In recent years,porphyrin-based MOFs constructed with porphyrins and their derivatives as organic ligands have attracted much attention.This material possesses not only the unique biological and chemical properties of porphyrin molecules,but also the advantages of MOFs themselves,showing excellent catalytic activity and optical properties.In this paper,some biosensors were designed and assembled based on the catalytic and fluorescent properties of porphyrin-based MOFs.The main contents are as follows:1.In this work,Glucose oxidase-immobilized metal–organic framework?GOD@Co-FeMOF?nanosheet system was developed as a tandem catalyst.In this system,glucose could be converted into gluconic acid and H₂O₂,and sequentially the latter could be used to catalyze the oxidation of L-arginine to generate nitric oxide in the presence of porphyrinic MOFs as artificial enzymes under physiological pH,showing great potential in cancer depleting glucose for starving-like/gas therapy.2.In this work,a novel bifunctional flower-like metal–organic framework?Cu-ZnMOF?was designed as a probe,which could be used for H₂S detection and in situ singlet-oxygen production.In the presence of H₂S,Cu-ZnMOF could be activated by H₂S,induce itself disassembly and then release linker,Zn porphyrin.Therefore,the fluorescence of the system is enhanced and ¹O₂.This biosensor shows the wide dynamic correlation of H₂S from 0.10 to 80?M,and the detection limit down to 35 nM,opening up a new avenue for selective H₂S detection under physiological pH and controllable ¹O₂release.3.This study reports a new artificial nanozyme based on ultra-small Pt nanoparticles?Pt NPs?grown on nanoscale metalloporphyrin metal organic frameworks?P-MOF?Fe???termed as Pt@P-MOF?Fe??as biomimetic catalysts and redox mediator to detect the telomerase activity.In this system,the P-MOF?Fe?were used as nanocarrier and signal media.The DNA functionalized Pt@P-MOF?Fe?was as signal probe and exhibited enhanced electrochemical signal in the presence of H₂O₂,owing to the synergistic effect between P-MOF?Fe?and Pt NPs.Upon the addition cell extract,the telomerase primer could extend and then hybridize with assistant DNA2 in the triple-helix,leading to the structure of triple-helix changes and release the hairpin DNA to hybridize with the capture DNA on the surface of Pt@P-MOF?Fe?,resulting in the electrochemical signal readout of H₂O₂ reduction.With the aid of recycling amplification of Exonuclease?,the telomeres sensor exhibited the detection down to 20 Hela cell mL^-1.This work supplies a new avenue to design artificial enzyme catalysts and serves as an ideal platform to use metalloporphyrin metal organic frameworks as signal media for detection of analytes.