Morphology Control And Application Of Metal Organic Hybrid Materials

Photothermotherapy?PTT?,as a minimally invasive and highly specific anti-tumor treatment,has attracted considerable attention in the past few years.The basic principle of photothermotherapy is to use photothermal agent?PTA?to convert light energy into heat energy under laser irradiation conditions,and then effectively kill cancer cells in the tumor area through the high heat effect in the body.To date,a variety of materials have been used in photothermal therapy.Among them,metal-organic hybrid materials have received widespread attention due to their good biocompatibility,biosafety and degradability.In this paper,two kinds of nano-sized metal-organic hybrid materials were synthesized,and their application was explored in photothermal treatment,as fllow:?1?Fe-o-nitro-p-phenylenediamine?Fe-PNPD?nanoparticles were first prepared by solution wet method.In order to improve the biocompatibility of Fe-PNPD nanoparticles,Fe-PNPD nanoparticles were modified with polyethylene glycol to obtain Fe-PNPD-PEG nanoparticles.The morphology and properties of Fe-PNPD-PEG nanoparticles were adjusted by changing the amount of organic ligands?o-nitro-p-phenylenediamine?and the types of solvents?methanol and water?.The morphology,particle size and uniformity of Fe-PNPD-PEG nanoparticles were determined by scanning electron microscopy?SEM?and transmission electron microscopy?TEM?.The structure and thermal stability of Fe-PNPD-PEG nanoparticles were determined by infrared spectroscopy?FTIR?,X-ray diffraction spectroscopy?XRD?,Zeta potential,X-ray photoelectron spectroscopy?XPS?and thermal weight loss.Through UV-Vis spectroscopy?UV?and infrared camera tracking tests,it was found that Fe-PNPD-PEG has strong absorption in the near-infrared region,and the temperature rises rapidly with time and the maximum temperature is stable under the irradiation.The cell experiments?cytocompatibility,endocytosis,cell death and cell flow experiments?and animal experiments?mouse weight monitoring,mouse tumor volume monitoring and mouse tumor dissection experiments?prove Fe-PNPD-PEG nano particles can efficiently suppress and eliminate tumor cel s.?2?Fe-p-aminophenol?Fe-PAP?nanoparticles were first prepared by a one-step method.In order to increase the biological targeting of Fe-PAP,the Fe-PAP nanoparticles were modified with hyaluronic acid to obtain HA-Fe-PAP nanoparticles.The morphology and properties of the nanoparticles were adjusted by changing the reaction temperature?60^oC and 80 ^oC?,the type of solvent?ethanol,dioxane,methanol,etc.?and the content of the reactant FeC l₃?0.01-0.09 mL?.The morphology,particle size and uniformity of HA-Fe-PAP nanoparticles were observed by SEM and TEM.The structure and thermal stability of HA-Fe-PAP nanoparticles were determined by FTIR,XRD,Zeta,XPS and TGA.Through UV and infrared camera tracking tests,it was found that HA-Fe-PAP nanoparticles have strong absorption in the near-infrared region,and HA-Fe-PAP nanoparticles have a rapid temperature increase over time under the irradiation of 808 nm laser.The maximum temperature is stable.The cell experiments?cytocompatibility,endocytosis,cell death and cell flow experiments?and animal experiments?mouse weight monitoring,mouse tumor volume monitoring and mouse tumor dissection experiments?prove Fe-PNPD-PEG nano particles can quickly and efficiently inhibit and eliminate tumor cel s.In summary,through a series of tests on these two nanoparticles,they were found to be excel ent photothermal agents,which can be used in photothermal therapy.