| Microfluidic technology has become one of the analysis technologies that have attracted widespread attention in recent years because of its low cost,low consumption,portability,integration,automation,and controllability.In past years,with the continuous popularization and development of smartphones,it has obvious advantages to use smartphones in analysis and detection.The purpose of this work is to establish a microfluidic detection platform based on the smartphone and use it for the detection of environmental pollutants and enzyme content in biological samples.Chapter 1: This chapter is mainly divided into three sections.The first section gives an overview of microfluidic technology,mainly introduces the development process of microfluidic technology,chip manufacturing materials,manufacturing methods,and its application in multiple fields such as food safety,disease diagnosis,etc.The second section gives an overview of the smartphone-based detection platform,mainly introducing the reasons why the smartphone can be used as a detection platform,its analysis method characteristics and the application of smartphone-based sensors.The third section summarizes colorimetric analysis based on smartphones and micro-fluidic chip,mainly introducing the application in the environment,food safety,and biological samples.Chapter 2: In this work,a low-cost,fast detection speed and portable microfluidic detection platform based on the smartphone is built.Based on the platform,an efficient and rapid method for determining the formaldehyde content in the air is established.Unlike most commercial formaldehyde detectors that use electronic sensor chips to determine the formaldehyde content,this work is based on the specific chemical color reaction between 4-amino-3-hydrazine-5-mercapto-1,2,4-triazathiol(AHMT)and formaldehyde.Through optimizing the reaction conditions,the optimal volume of AHMT is 50 ?L,the optimal dosage of potassium periodate is15 ?L,the optimal reaction time is 5 min.Using the smartphone and microfluidic chip to detect the formaldehyde content in the air,the linear range is 62.6 mg/L–132 mg/L,and the detection limit is 2.33 mg/L.In addition,the detection of the same solution by using different types of mobile phones has proved the universality of the method.And compared with national standard detection methods,the results are consistent.Chapter 3: This article optimizes the self-built low-cost smartphone detection equipment and microfluidic chip.By investigating the channel shape,length,and pretreatment method of the microfluidic chip and their influence on the detection sensitivity,the pretreatment method,channel shape,and length of the microfluidic chip with the best detection effect are determined.The lens is used to improve the detached detection platform used in the previous work into an integrated detection device,and its detection effect is not affected.Subsequently,the performance of the optimized detection platform is evaluated,and the results show that its detection sensitivity is improved,and the color of the solution has no effect on its detection effect.The detection equipment can be used for the colorimetric detection of different substances.Chapter 4: The activity of alkaline phosphatase(ALP)is an indicator of many diseases.Measuring the ALP activity in serum can determine the health status of an individual.In this paper,the ALP activity was determined by the self-built integrated detection platform.This work is based on the fact that p-NPP can be catalyzed by ALP into p-nitrophenol under alkaline conditions,and the reaction conditions are optimized,which are 1.2 mg/mL for substrate concentration,15 min for reaction time,and 50?L for the optimal stop solution dosage(that is sodium hydroxide solution).The alkaline phosphatase in human serum is detected with the smartphone and microfluidic chip,the linear range is 0.132 mmol/L-0.992 mmol/L,and the detection limit is 9.13?mol/L.The test results are consistent with commercial kit. |
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