Studies On Phosphorescent Chemosensors For Peroxynitrite Based On Iridium(?) And Ruthenium(?) Complexes

Phosphorescent metal complex-based chemosensors with the unique excellent optical properties such as larger Stokes shift,longer lumincence life and strong photobleaching resistance have received extensive attention in the field of molecule or ion detection,biomarker imaging,disease markers detection and so on.On the other hand,as one of the endogenous reactive oxygen species(ROS),peroxynitrite(ONOO~-)plays an important role in cell signaling and metabolism.The abnormal content of ONOO~-often could lead to many diseases.Therefore,in the perspective of luminescent materials,phosphorescent metal complex-based chemosensors could make up for the deficiencies of existing fluorescent sensors in tracking and detecting the content of ONOO~-in the body,further improving the accuracy and sensitivity in detection.In this dissertation,a type of phosphorescent iridium(?)and ruthenium(?)complex-based chemosensors have been designed to detect ONOO~-through the change of optical signals.This dissertation mainly includes the following three parts:Chapter 1:The structures,basic properties and practical applications of phosphorescent complex-based chemosensors,especially iridium(?)and ruthenium(?)complex-based chemosensors,have been concisely introduced.At the same time,the research progress and recognition mechanism of photochemical chemosensors to detect ONOO~-have been summarized.The advantages and applications of these phosphorescent chemosensors in the detection of ONOO~-were exhibited.Chapter 2:By means of the specific reaction of ONOO~-with?-ketoamide,we designed and synthesized a phosphorescent ruthenium(?)complex chemosensor for detecting ONOO~-.The chemosensor exhibited fast response,high selectivity and red phosphorescence enhancement to ONOO~-.Combined with time-resolved luminescence technology,the chemosensor with long phosphorescence lifetime could successfully remove the interference of background fluorescence.Cell imaging results showed that the sensor could successfully enter the living cells to detect endogenous ONOO~-.In addition,ruthenium(?)complex sensor possessed a larger two-photon absorption cross section,which could broaden its application in ONOO~-imaging.Chapter 3:By changing the central metal,we synthesized another phosphorescent iridium(?)complex-based chemosensor for the detection of ONOO~-.The sensor showed yellow phosphorescence enhancement towards ONOO~-,and also had high selectivity and specificity.The iridium(?)complex chemosensor could be located in the mitochondria in cells and successfully image endogenous ONOO~-by two-photon laser confocal microscopy.At the same time,we had further studied the process of drug-induced hepatotoxicity with the production of ONOO~-,which was expected to provide useful information for further pathological and pharmacological research.